ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7275) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ST3 beta -Gal a-2,3-Sialyltransferase 2/ST3GAL2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2
  Pro52-Gln350
  Accession # NP_008858
• Specificity: Detects human, mouse and rat ST3 beta -Gal alpha -2,3-Sialyltransferase 2/ST3GAL2 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ST6GALNAC4 and rhST3GAL1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ST3GAL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody

• Alpha 2,3-ST 2
• Beta-galactoside alpha-2,3-sialyltransferase 2
• beta-galactoside alpha-2,3-sialytransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2
• EC 2.4.99.4
• EC 2.4.99.7
• Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
• Gal-NAc6S
• sialyltransferase 4B (beta-galactosidase alpha-2,3-sialytransferase)
• Sialyltransferase 4B
• SIAT4B
• SIAT4-B
• ST3 beta-galactoside alpha-2,3-sialyltransferase 2
• ST3GAL2
• ST3GalA.2
• ST3GalA.2ST3Gal II
• ST3GALII

Background

Sialyltransferases add sialic acid to glycoproteins or glycosphingolipids and play important roles in many biological processes including immune recognition, pathogen infection, and cell adhesion (1). Similar to ST3GAL1 (2), ST3GAL2 is a type II membrane protein localized in the trans-Golgi network that can transfer sialic acid to the Gal beta 1-3GalNAc structure to form NeuAc alpha 2-3Gal beta 1-3GalNAc found in terminal carbohydrate groups of particular glycoproteins, oligosaccharides and glycolipids (3). Unlike the ubiquitously expressed ST3GAL1 (4), ST3GAL2 is mainly expressed in heart, liver, skeletal muscle and various lymphoid tissues but not in brain and kidney (3). ST3GAL2 is responsible for the synthesis of monosialosyl globopentaosylceramide (MSGb5), also known as stage-specific embryonic antigen-4 (SSEA4) (5), a cell surface marker for testicular germ cell carcinoma, renal cell carcinoma, and mesenchymal stem cells (6). The enzymatic activity of recombinant human ST3GAL2 was determined using a phosphatase-coupled glycosyltransferase assay (7). Over aa 52-350 human ST3GAL2 share 94% aa sequence identity with mouse and rat.

1. Varki, A. (1999) Glycobiology 2:25.
2. Jeanneau, C. et al. (2004) J. Biol. Chem. 279:13461.
3. Giordanengo, V. et al. (1997) Eur. J. Biochem. 247:558.
4. Kitagawa, H. and Paulson, J.C. (1994) J. Biol. Chem. 269:17872.
5. Saito, S. et al. (2003) J. Biol. Chem. 278:26474.
6. Suila, H. et al. (2011) J. Mol. Cell Biol. 3:99.
7. Wu, Z.L. et al. (2011) Glycobiology 21:727.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST3 beta-Gal alpha-2,3-Sialyltransferase 2/ST3GAL2 Antibody (AF7275)(1)

Publication using AF7275

• TR Karhadkar, D Pilling, N Cox, RH Gomer Sialidase inhibitors attenuate pulmonary fibrosis in a mouse model Sci Rep, 2017-11-08;7(1):15069. 2017-11-08 [PMID: 29118338] (IHC-Fr, Human)

Bioinformatics

• Gene Symbol: ST3GAL2
• Uniprot:
  • NP_008858()