ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Gal Sialyltransferase 1/ST6GAL1
Lys27-Cys406
Accession # P15907
Specificity
Detects human Gal Sialyltransferase 1/ST6GAL1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ST6GAL1
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF5924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

  • Alpha 2,6-ST
  • B-cell antigen CD75
  • beta-galactoside alpha-2,6-sialyltransferase 1
  • CD75
  • CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
  • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
  • EC 2.4.99.1
  • MGC48859
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
  • Sialyltransferase 1
  • SIAT1
  • SIAT1Alpha 2,6-ST 1
  • ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
  • ST6Gal I
  • ST6GAL1
  • ST6GalI
  • ST6N

Background

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

  1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
  2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
  4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
  5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
  6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
  7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
  8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  9. Kitazume, S. et al. (2009) Glycobiology 19:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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AF5924
Species: Hu
Applications: WB, Simple Western

Publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (AF5924)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 3 applications: ICC, IHC-Fr, Western Blot.


Filter By Application
ICC
(3)
IHC-Fr
(1)
Western Blot
(8)
All Applications
Filter By Species
Human
(10)
Mouse
(1)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF5924 Applications Species
KA Dorsett, RB Jones, KE Ankenbauer, AB Hjelmeland, SL Bellis Sox2 promotes expression of the ST6Gal-I glycosyltransferase in ovarian cancer cells J Ovarian Res, 2019;12(1):93. 2019 [PMID: 31610800] (Western Blot, Human) Western Blot Human
Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey Transfer of Functional Cargo in Exomeres Cell Rep, 2019;0(0):. 2019 [PMID: 30956133] (Western Blot, Human) Western Blot Human
J Mayr, K Lau, JCC Lai, IA Gagarinov, Y Shi, S McAtamney, RWY Chan, J Nicholls, M von Itzste, T Haselhorst Unravelling the Role of O-glycans in Influenza A Virus Infection Sci Rep, 2018;8(1):16382. 2018 [PMID: 30401951] (ICC, Human) ICC Human
MA Schulz, W Tian, Y Mao, J Van Coilli, L Sun, JS Larsen, YH Chen, C Kristensen, SY Vakhrushev, H Clausen, Z Yang Glycoengineering design options for IgG1 in CHO cells using precise gene editing Glycobiology, 2018;0(0):. 2018 [PMID: 29596681] (ICC, Hamster) ICC Hamster
RB Jones, KA Dorsett, AB Hjelmeland, SL Bellis The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29475939] (Western Blot, Human) Western Blot Human
CM Britain, AT Holdbrooks, JC Anderson, CD Willey, SL Bellis Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death J Ovarian Res, 2018;11(1):12. 2018 [PMID: 29402301] (Western Blot, Human) Western Blot Human
AT Holdbrooks, CM Britain, SL Bellis ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29233887] (Western Blot, Human) Western Blot Human
A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29191829] (Western Blot, Human) Western Blot Human
Mark B Jones B-cell-independent sialylation of IgG Proc Natl Acad Sci USA, 2016;113(26):7207-12. 2016 [PMID: 27303031] (IHC-Fr, Mouse) IHC-Fr Mouse
The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype Cancer Res, 2016;0(0):. 2016 [PMID: 27216178] (Western Blot, Human) Western Blot Human
Show All 13 Publications.

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Biotin BAF5924

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Bioinformatics

Gene Symbol ST6GAL1
Uniprot