ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by ...read more
ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified ...read more
ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using ...read more
ST6Gal-I expression in OV4 ovarian cancer cells. OV4 cells that have no endogenous ST6Gal-I were stably transduced with either empty vector or ST6Gal-I-expressing lentivirus. ST6Gal-I expression was confirmed by ...read more
ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed U266 human myeloma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative) using Goat ...read more
ST6Gal-I expression in OV4 ovarian cancer cells. OV4 cells that have no endogenous ST6Gal-I were stably transduced with either empty vector or ST6Gal-I-expressing lentivirus. ST6Gal-I expression was confirmed by ...read more
Cells that are resistant to cisplatin have upregulated endogenous ST6Gal-I. (A) Pa-1 cells with ST6Gal-I knockdown (sh.ST6) were exposed to cisplatin for 3 weeks, and the remaining viable population (sh.ST6 cis-res) ...read more
Cells that are resistant to cisplatin have upregulated endogenous ST6Gal-I. (A) Pa-1 cells with ST6Gal-I knockdown (sh.ST6) were exposed to cisplatin for 3 weeks, and the remaining viable population (sh.ST6 cis-res) ...read more
Cells that are resistant to cisplatin have upregulated endogenous ST6Gal-I. (A) Pa-1 cells with ST6Gal-I knockdown (sh.ST6) were exposed to cisplatin for 3 weeks, and the remaining viable population (sh.ST6 cis-res) ...read more
ST6Gal-I expression in OV4 ovarian cancer cells. OV4 cells that have no endogenous ST6Gal-I were stably transduced with either empty vector or ST6Gal-I-expressing lentivirus. ST6Gal-I expression was confirmed by ...read more
ST6Gal-I knockdown in Pa-1 ovarian cancer cells. Pa-1 cells that have high endogenous ST6Gal-I were stably transduced with either empty vector lentivirus, or virus expressing shRNA for ST6Gal-I. ST6Gal-I knockdown was ...read more
ST6Gal-I knockdown in Pa-1 ovarian cancer cells. Pa-1 cells that have high endogenous ST6Gal-I were stably transduced with either empty vector lentivirus, or virus expressing shRNA for ST6Gal-I. ST6Gal-I knockdown was ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ST6 Gal Sialyltransferase 1/ST6GAL1
Lys27-Cys406
Accession # P15907
Specificity
Detects human ST6 Gal Sialyltransferase 1/ST6GAL1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ST6GAL1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publications using
AF5924 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

  • Alpha 2,6-ST
  • B-cell antigen CD75
  • beta-galactoside alpha-2,6-sialyltransferase 1
  • CD75
  • CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
  • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
  • EC 2.4.99.1
  • MGC48859
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
  • sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
  • Sialyltransferase 1
  • SIAT1
  • SIAT1Alpha 2,6-ST 1
  • ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
  • ST6Gal I
  • ST6GAL1
  • ST6GalI
  • ST6N

Background

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

  1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
  2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
  4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
  5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
  6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
  7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
  8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
  9. Kitazume, S. et al. (2009) Glycobiology 19:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (AF5924)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Hamster.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(1)
ICC
(5)
IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(16)
All Applications
Filter By Species
Human
(20)
Mouse
(2)
Hamster
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using AF5924 Applications Species
Dashzeveg, NK;Jia, Y;Zhang, Y;Gerratana, L;Patel, P;Shajahan, A;Dandar, T;Ramos, EK;Almubarak, HF;Adorno-Cruz, V;Taftaf, R;Schuster, EJ;Scholten, D;Sokolowski, MT;Reduzzi, C;El-Shennawy, L;Hoffmann, AD;Manai, M;Zhang, Q;D'Amico, P;Azadi, P;Colley, KJ;Platanias, LC;Shah, AN;Gradishar, WJ;Cristofanilli, M;Muller, WA;Cobb, BA;Liu, H; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer Cancer discovery 2023-06-05 [PMID: 37272843] (Western Blot, Human) Western Blot Human
J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID- The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees EBioMedicine, 2022-12-16;87(0):104408. 2022-12-16 [PMID: 36529104] (Flow Cytometry, Human) Flow Cytometry Human
X Zou, J Lu, Y Deng, Q Liu, X Yan, Y Cui, X Xiao, M Fang, F Yang, H Sawaki, T Sato, B Tan, X Lu, B Feng, A Kuno, H Narimatsu, C Gao, Y Zhang ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface Oncogene, 2022-12-17;0(0):. 2022-12-17 [PMID: 36528750] (IHC, Human) IHC Human
Natallia Makarava, Elizaveta Katorcha, Jennifer Chen-Yu Chang, Joseph T. Y. Lau, Ilia V. Baskakov Deficiency in ST6GAL1, one of the two alpha 2,6-sialyltransferases, has only a minor effect on the pathogenesis of prion disease Frontiers in Molecular Biosciences 2022-11-14 [PMID: 36452458]
S Gc, K Tuy, L Rickenback, R Jones, A Chakrabort, CR Miller, EA Beierle, VS Hanumanthu, AN Tran, JA Mobley, SL Bellis, AB Hjelmeland alpha2,6 Sialylation mediated by ST6GAL1 promotes glioblastoma growth JCI Insight, 2022-11-08;7(21):. 2022-11-08 [PMID: 36345944] (IHC, Western Blot, Human) IHC, Western Blot Human
NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness Cancer Gene Therapy, 2022-06-08;0(0):. 2022-06-08 [PMID: 35676533] (Western Blot, Human) Western Blot Human
Mingfeng Zhang, Tong Qi, Lu Yang, Daniel Kolarich, Nora Heisterkamp Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia Frontiers in Oncology 2022-03-16 [PMID: 35371997]
M Smithson, R Irwin, G Williams, KL Alexander, LE Smythies, M Nearing, MC McLeod, S Al Diffalh, SL Bellis, KM Hardiman Sialyltransferase ST6GAL-1 mediates resistance to chemoradiation in rectal cancer The Journal of Biological Chemistry, 2022-01-15;0(0):101594. 2022-01-15 [PMID: 35041825] (IHC, Western Blot, Human) IHC, Western Blot Human
AT Holdbrooks, KE Ankenbauer, J Hwang, SL Bellis Regulation of inflammatory signaling by the ST6Gal-I sialyltransferase PLoS ONE, 2020-11-09;15(11):e0241850. 2020-11-09 [PMID: 33166339] (Western Blot, Mouse) Western Blot Mouse
CM Britain, N Bhalerao, AD Silva, A Chakrabort, DJ Buchsbaum, MR Crowley, DK Crossman, YJK Edwards, SL Bellis Glycosyltransferase ST6Gal-I promotes the epithelial to mesenchymal transition in pancreatic cancer cells J Biol Chem, 2020-11-23;0(0):. 2020-11-23 [PMID: 33148698] (Western Blot, Human) Western Blot Human
Show All 28 Publications.

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Bioinformatics

Gene Symbol ST6GAL1
Uniprot