ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ST6GAL1 at approximately 56 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for ST6 Gal Sialyltransferase 1/ST6GAL1 at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

ST6 Gal Sialyltransferase 1/ST6GAL1 was detected in immersion fixed U266 human myeloma cell line (Positive) & RT‑4 human urinary bladder transitional cell papilloma cell line (Negative) using Goat Anti-Human ST6 Gal Sialyltransferase 1/ST6GAL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5924) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to Golgi. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ST6 Gal Sialyltransferase 1/ST6GAL1
  Lys27-Cys406
  Accession # P15907
• Specificity: Detects human ST6 Gal Sialyltransferase 1/ST6GAL1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ST6GAL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 0.3-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [Unconjugated]

• Alpha 2,6-ST
• B-cell antigen CD75
• beta-galactoside alpha-2,6-sialyltransferase 1
• CD75
• CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
• EC 2.4.99.1
• MGC48859
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
• Sialyltransferase 1
• SIAT1
• SIAT1Alpha 2,6-ST 1
• ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• ST6Gal I
• ST6GAL1
• ST6GalI
• ST6N

Background

Sialic acid molecules attached to glycoproteins or glycosphingolipids play important roles in various biological processes such as immune recognition, pathogen infection, and cell adhesion (1). Sialyltransferases are key enzymes that regulate the cellular levels of sialic acid-containing molecules. Beta-galactosamide alpha‑2,6‑sialyltransferase 1 encoded by the ST6GAL1 gene is a type-II membrane protein localized in the trans-Golgi network and catalyzes 2,6-sialylation of Gal beta 1,4‑GlcNAc structures on N-glycans (2). The enzyme is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB‑6, CD75, and CD76 (3). ST6GAL1 is highly expressed in the liver and also expressed in most other tissues to some extent (4). ST6GAL1 deficiency causes abnormalities in B-cell immunoreactivity (5). The expression and activity of ST6GAL1 are associated with tumor metastasis in breast (6) and colon (7) cancers. The majority of ST6GAL1 in the liver is cleaved and secreted into the serum (8) and may be used as a biomarker for hepatitis diseases (9).

1. Varki, A. et al. (1999) Essentials of Glycobiology, Cold Spring Harbor Laboratory Press, pp195.
2. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
3. Bast, B.J. et al. (1992) J. Cell Biol. 116:423.
4. Kitagawa, H. and J.C. Paulson (1994) J. Biol. Chem. 269:17872.
5. Hennet, T. et al. (1998) Proc. Natl. Acad. Sci. USA 95:4504.
6. Recchi, M.A. et al. (1998) Cancer Res. 58:4066.
7. Dall’Olio, F. et al. (2001) Eur. J. Biochem. 268:5876.
8. Weinstein, J. et al. (1987) J. Biol. Chem. 262:17735.
9. Kitazume, S. et al. (2009) Glycobiology 19:479.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody (AF5924)(21)

Publications using AF5924

• J Van Coilli, T Pongracz, J Rahmöller, HJ Chen, CE Geyer, LA van Vught, JS Buhre, T Šušti?, TLJ van Osch, M Steenhuis, W Hoepel, W Wang, AS Lixenfeld, J Nouta, S Keijzer, F Linty, R Visser, MD Larsen, EL Martin, I Künsting, S Lehrian, V von Kopylo, C Kern, HB Lunding, M de Winther, N van Mourik, T Rispens, T Graf, MA Slim, RP Minnaar, MK Bomers, JJ Sikkens, APJ Vlaar, CE van der Sc, J den Dunnen, M Wuhrer, M Ehlers, G Vidarsson, Fatebenefr, UMC COVID- The BNT162b2 mRNA SARS-CoV-2 vaccine induces transient afucosylated IgG1 in naive but not in antigen-experienced vaccinees EBioMedicine, 2022;87(0):104408. 2022 [PMID: 36529104] (Flow Cytometry, Human)
• X Zou, J Lu, Y Deng, Q Liu, X Yan, Y Cui, X Xiao, M Fang, F Yang, H Sawaki, T Sato, B Tan, X Lu, B Feng, A Kuno, H Narimatsu, C Gao, Y Zhang ST6GAL1 inhibits metastasis of hepatocellular carcinoma via modulating sialylation of MCAM on cell surface Oncogene, 2022;0(0):. 2022 [PMID: 36528750] (IHC, Human)
• NC Hait, A Maiti, R Wu, VL Andersen, CC Hsu, Y Wu, DG Chapla, K Takabe, ME Rusiniak, W Bshara, J Zhang, KW Moremen, JTY Lau Extracellular sialyltransferase st6gal1 in breast tumor cell growth and invasiveness Cancer Gene Therapy, 2022;0(0):. 2022 [PMID: 35676533] (Western Blot, Human)
• M Smithson, R Irwin, G Williams, KL Alexander, LE Smythies, M Nearing, MC McLeod, S Al Diffalh, SL Bellis, KM Hardiman Sialyltransferase ST6GAL-1 mediates resistance to chemoradiation in rectal cancer The Journal of Biological Chemistry, 2022;0(0):101594. 2022 [PMID: 35041825] (Western Blot, Human)
• AT Holdbrooks, KE Ankenbauer, J Hwang, SL Bellis Regulation of inflammatory signaling by the ST6Gal-I sialyltransferase PLoS ONE, 2020;15(11):e0241850. 2020 [PMID: 33166339] (Western Blot, Mouse)
• CM Britain, N Bhalerao, AD Silva, A Chakrabort, DJ Buchsbaum, MR Crowley, DK Crossman, YJK Edwards, SL Bellis Glycosyltransferase ST6Gal-I promotes the epithelial to mesenchymal transition in pancreatic cancer cells J Biol Chem, 2020;0(0):. 2020 [PMID: 33148698] (Western Blot, Human)
• KL Alexander, CA Serrano, A Chakrabort, M Nearing, LN Council, A Riquelme, M Garrido, SL Bellis, LE Smythies, PD Smith Modulation of glycosyltransferase ST6Gal-I in gastric cancer-derived organoids disrupts homeostatic epithelial cell turnover J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32763973] (ICC, Human)
• L Ou, X He, N Liu, Y Song, J Li, L Gao, X Huang, Z Deng, X Wang, S Lin Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance Mol Med Rep, 2020;21(3):1449-1460. 2020 [PMID: 32016470] (Western Blot, Human)
• KA Dorsett, RB Jones, KE Ankenbauer, AB Hjelmeland, SL Bellis Sox2 promotes expression of the ST6Gal-I glycosyltransferase in ovarian cancer cells J Ovarian Res, 2019;12(1):93. 2019 [PMID: 31610800] (Western Blot, Human)
• Q Zhang, JN Higginboth, DK Jeppesen, YP Yang, W Li, ET McKinley, R Graves-Dea, J Ping, CM Britain, KA Dorsett, CL Hartman, DA Ford, RM Allen, KC Vickers, Q Liu, JL Franklin, SL Bellis, RJ Coffey Transfer of Functional Cargo in Exomeres Cell Rep, 2019;0(0):. 2019 [PMID: 30956133] (Western Blot, Human)
• J Mayr, K Lau, JCC Lai, IA Gagarinov, Y Shi, S McAtamney, RWY Chan, J Nicholls, M von Itzste, T Haselhorst Unravelling the Role of O-glycans in Influenza A Virus Infection Sci Rep, 2018;8(1):16382. 2018 [PMID: 30401951] (ICC, Human)
• MA Schulz, W Tian, Y Mao, J Van Coilli, L Sun, JS Larsen, YH Chen, C Kristensen, SY Vakhrushev, H Clausen, Z Yang Glycoengineering design options for IgG1 in CHO cells using precise gene editing Glycobiology, 2018;0(0):. 2018 [PMID: 29596681] (ICC, Hamster)
• RB Jones, KA Dorsett, AB Hjelmeland, SL Bellis The ST6Gal-I sialyltransferase protects tumor cells against hypoxia by enhancing HIF-1? signaling J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29475939] (Western Blot, Human)
• CM Britain, AT Holdbrooks, JC Anderson, CD Willey, SL Bellis Sialylation of EGFR by the ST6Gal-I sialyltransferase promotes EGFR activation and resistance to gefitinib-mediated cell death J Ovarian Res, 2018;11(1):12. 2018 [PMID: 29402301] (Western Blot, Human)
• AT Holdbrooks, CM Britain, SL Bellis ST6Gal-I sialyltransferase promotes tumor necrosis factor (TNF)-mediated cancer cell survival via sialylation of the TNF receptor 1 (TNFR1) death receptor J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29233887] (Western Blot, Human)
• A Chakrabort, KA Dorsett, HQ Trummell, ES Yang, PG Oliver, JA Bonner, DJ Buchsbaum, SL Bellis ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage J. Biol. Chem., 2017;0(0):. 2017 [PMID: 29191829] (Western Blot, Human)
• Mark B Jones B-cell-independent sialylation of IgG Proc Natl Acad Sci USA, 2016;113(26):7207-12. 2016 [PMID: 27303031] (IHC-Fr, Mouse)
• The tumor-associated glycosyltransferase ST6Gal-I regulates stem cell transcription factors and confers a cancer stem cell phenotype Cancer Res, 2016;0(0):. 2016 [PMID: 27216178] (Western Blot, Human)
• Suzuki O, Abe M Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: regulatory roles of cell surface glycans. Int J Oncol, 2014;44(5):1433-42. 2014 [PMID: 24589677] (ICC, Human)
• Schultz , Matthew, Swindall , Amanda F, Wright , John W, Sztul , Elizabet, Landen , Charles, Bellis , Susan L ST6Gal-I sialyltransferase confers cisplatin resistance in ovarian tumor cells. J Ovarian Res, 2013;6(1):25. 2013 [PMID: 23578204]
• Swindall A, Londono-Joshi A, Schultz M, Fineberg N, Buchsbaum D, Bellis S ST6Gal-I protein expression is upregulated in human epithelial tumors and correlates with stem cell markers in normal tissues and colon cancer cell lines. Cancer Res, 2013;73(7):2368-78. 2013 [PMID: 23358684] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ST6GAL1
• Uniprot:
  • P15907()