ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-31449] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-31449] - Human ovarian cancer.SIAT4A stained by SIAT4A antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-31449] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIAT4A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody [NBP1-31449] -Human tissue extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIAT4A antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu, Pm, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A. The exact sequence is proprietary.
• Localization: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein, Secreted
• Predicted Species: Chimpanzee (99%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST3GAL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for ST3 beta-Gal alpha-2,3-Sialyltransferase 1/ST3GAL1/SIAT4A Antibody - Azide and BSA Free

• Alpha 2,3-ST 1
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 1
• DKFZp666E036
• DKFZp779K2051
• EC 2.4.99
• EC 2.4.99.4
• Gal-beta-1,3-GalNAc-alpha-2,3-sialyltransferase
• Gal-NAc6S
• sialyltransferase 4A (beta-galactosidase alpha-2,3-sialytransferase)
• sialyltransferase 4A (beta-galactoside alpha-2,3-sialyltransferase)
• sialyltransferase 4A (beta-galactoside alpha-2,3-sialytransferase)
• Sialyltransferase 4A
• SIAT4
• SIAT4A
• SIAT4-A
• SIATFL
• SIATFLFLJ36548
• ST3 beta-galactoside alpha-2,3-sialyltransferase 1
• ST3Gal I
• ST3GAL1
• ST3GalA
• ST3GalA.1MGC9183
• ST3GalI
• ST3GalIA
• ST3GalIA1,Beta-galactoside alpha-2,3-sialyltransferase 1
• ST3O
• ST3OST3GalA

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ST3GAL1