ST2/IL-33R Antibody (245714) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB
• Clone: 245714
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

ST2/IL-33R Antibody (245714) [Alexa Fluor® 532] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ST2/IL-33 R
  Ser27-Arg332
  Accession # P14719
• Specificity: Detects mouse ST2/IL-33 R in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human ST2 and no cross-reactivity with recombinant mouse (rm) IL-1 R1 or rmIL-1 R2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST2/IL-33R Antibody (245714) [Alexa Fluor® 532]

• DER4
• DER4ST2growth stimulation-expressed
• Fit-1
• IL-1 R4
• IL1R4
• IL-1R4
• IL-1RL1
• IL-33 R
• IL33R
• IL-33R
• interleukin 1 receptor-like 1
• interleukin 1 receptor-related protein
• interleukin-1 receptor-like 1
• Ly84
• MGC32623
• Protein ST2
• ST2
• ST2L
• ST2V
• T1
• T1homolog of mouse growth stimulation-expressed

Background

ST2, also known as IL-33R, IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4‑7). It binds IL-33, a cytokine that is up‑regulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11‑13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33's ability to signal through transmembrane ST2 (9, 12‑14).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.