SSBP1 Antibody - BSA Free

Western Blot: SSBP1 Antibody [NBP2-95237] - Analysis of extracts of Mouse liver, using SSBP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. xposure time: 90s.

Western Blot: SSBP1 Antibody [NBP2-95237] - Analysis of extracts of various cell lines, using SSBP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.

Immunoprecipitation: SSBP1 Antibody [NBP2-95237] - Analysis of 200ug extracts of MCF7 cells using 1ug SSBP1 antibody. Western blot was performed from the immunoprecipitate using SSBP1 antibody at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] - Confocal immunofluorescence analysis of U2OS cells using SSBP1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SSBP1 Antibody - BSA Free [SSBP1] - Immunofluorescence analysis of U2OS cells using SSBP1 Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SSBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SSBP1 Antibody - BSA Free (NBP2-95237) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1). MFRRPVLQVLRQFVRHESETTTSLVLERSLNRVHLLGRVGQDPVLRQVEGKNPVTIFSLATNEMWRSGDSEVYQLGDVSQKTTWHRISVFRPGLRDVAYQYVKKGSRIYLEGKIDYGEYMDKNNVRRQATTIIADNIIFLSDQTKEKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSBP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SSBP1 Antibody - BSA Free

• mitochondrial
• MtSSB
• single-stranded DNA binding protein 1
• SSBP1

Background

The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Mitochondrial single-stranded DNA-binding protein (mtSSB or SSBP1) is necessary for mtDNA replication. SSBP1 is a homotetramer that binds preferentially and cooperatively to single-stranded DNA. It is localized to the mitochondria in normal and neoplastic human tissue of different origin, function, and differentiation, and is likely involved in mitochondrial DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSBP1