SRPK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SRPK1 Antibody [NBP1-87050] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Analysis using Anti-SRPK1 antibody NBP1-87050 (A) shows similar pattern to independent antibody NBP2-58538 (B).

Analysis in control (vector only transfected HEK293T lysate) and SRPK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRPK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SRPK1 Antibody - BSA Free (NBP1-87050) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRPK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SRPK1 Protein (NBP1-87050PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SRPK1 Antibody - BSA Free

• EC 2.7.11.1
• Serine/arginine-rich protein-specific kinase 1
• serine/arginine-rich splicing factor kinase 1
• serine/threonine-protein kinase SRPK1
• SFRS protein kinase 1SFRSK1
• SR protein kinase 1
• SRPK1
• SR-protein-specific kinase 1
• SRSF protein kinase 1

Background

SRPK1 encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRPK1