SRPK2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SRPK2 Antibody [NBP1-87049] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, nucleoli & cytosol.

Staining of human skeletal muscle shows moderate nuclear positivity in subset of myocytes.

Staining of human cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.

Staining of human stomach shows no positivity in glandular cells as expected.

Staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neurons.

Analysis in control (vector only transfected HEK293T lysate) and SRPK2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SRPK2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SRPK2 Antibody - BSA Free (NBP1-87049) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: QQLDDEDDDEEDCPNPEEYNLDEPNAESDYTYSSSYEQFNGELPNGRHKIPESQFPEFSTSLFSGSLEPVACGSVLSEGSPLTEQEESSPSHDRSRTVSASSTGDLPKAKTRAADLLVNPL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRPK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SRPK2 Protein (NBP1-87049PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SRPK2 Antibody - BSA Free

• EC 2.7.11.1
• FLJ36101
• H_RG152G17.1a
• H_RG152G17.1b
• serine kinase SRPK2
• Serine/arginine-rich protein-specific kinase 2
• serine/arginine-rich splicing factor kinase 2
• serine/threonine-protein kinase SRPK2
• SFRS protein kinase 2SFRSK2
• SFRSK2
• SR protein kinase 2
• SRPK2
• SR-protein-specific kinase 2
• SRSF protein kinase 2
• WUGSC:H_RG152G17.1a

Background

Serine/arginine rich protein-specific kinase 2 (SRPK2) phosphorylates RS domain-containing proteins, such as SFRS1 and SFRS2 on serine residues. SRPK2 plays a role in spliceosome assembly and in mediating the trafficking of splicing factors. SRPK2 also appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRPK2