Western Blot: TOR/mTOR Antibody [NB100-240] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-mTOR antibody used for WB at 0.2 ug/ml. ...read more
Independent Antibodies: Immunoprecipitation: TOR/mTOR Antibody [NB100-240] - Detection of human mTOR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa ...read more
Rat reactivity reported in scientific literature (PMID: 29728793).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for TOR/mTOR Antibody
FK506 binding protein 12-rapamycin associated protein 1
FK506 binding protein 12-rapamycin associated protein 2
FK506-binding protein 12-rapamycin complex-associated protein 1
FKBP12-rapamycin complex-associated protein
FRAP1FKBP12-rapamycin complex-associated protein 1
Mammalian target of rapamycin
mechanistic target of rapamycin (serine/threonine kinase)
Mechanistic target of rapamycin
rapamycin and FKBP12 target 1
rapamycin associated protein FRAP2
Rapamycin target protein 1
RAPT1FKBP-rapamycin associated protein
serine/threonine-protein kinase mTOR
FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Suzuki H, Roa JC, Kawamoto T. Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients MOLECULAR AND CLINICAL ONCOLOGY 2015 Jan 09 (IHC-P, Human)
Details: TOR/mTOR antibody used for IHC-P on tissue specimens obtained from patients having carcinomas of the gallbladder /GBCa, extrahepatic bile duct carcinoma /EHBDCa and intrahepatic bile duct carcinoma /IHBDCa. IHC assay involved antigen retrieval with 10 mM sodium citrate buffer pH 6 employing high power of microwave treatment for 10 minutes and the primary antibody was used at 1:100 dilution with 4C ON incubation which followed labelling with polymer-horseradish peroxidase and DAB detection (Figure 1).
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