SRFBP1 Antibody - Azide and BSA Free

Immunoprecipitation: SRFBP1 Antibody [NBP3-00297] - SRFBP1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 2 uL NBP3-00297 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00297, at 1:100 dilution. Secondary antibody: IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 68 kDa

Western Blot: SRFBP1 Antibody [NBP3-00297] - NBP3-00297 at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:47 kDa Observed band size:68 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SRFBP1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human SRFBP1 (Uniprot#: Q8NEF9; Met1-Asp429)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRFBP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for SRFBP1 Antibody - Azide and BSA Free

• BUD22 homolog
• BUD22
• FLJ25286
• P49
• p49/STRAP
• Rlb1
• serum response factor binding protein 1
• serum response factor-binding protein 1
• SRF-dependent transcription regulation associated protein
• SRF-dependent transcription regulation-associated protein
• STRAP

Background

SRFBP1 may be involved in regulating transcriptional activation of cardiac genes during the aging process. May playa role in biosynthesis and/or processing of SLC2A4 in adiposecells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SRFBP1