SRD5A2 Antibody

Images

 
Western Blot: SRD5A2 Antibody [NBP1-59525] - HepG2 cell lysate, concentration 0.2-1 ug/ml.
Western Blot: SRD5A2 Antibody [NBP1-59525] - Lanes: Lane 1 : 50ug monkey brain extract Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti rabbit-HRP Secondary Antibody Dilution: 1 : 10,000.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SRD5A2 Antibody Summary

Immunogen
Synthetic peptides corresponding to SRD5A2 (steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 2)) The peptide sequence was selected from the N terminal of SRD5A2)(50ug). Peptide sequence KHTES
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRD5A2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against SRD5A2 and was validated on Western blot.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for SRD5A2 Antibody

  • 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
  • EC 1.3.99.5
  • MGC138457
  • S5AR 2
  • SR type 2
  • Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
  • steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
  • Type II 5-alpha reductase

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SRD5A2
Uniprot