SRD5A2 Antibody

Western Blot: SRD5A2 Antibody [NBP1-46510] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:4000 incubated at 4C overnight.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using using HRP polymer following manufacturers inctructions. DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer from Leica following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP polymer following manufacturers instructions; DAB chromogen. Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer following manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP polymer from manufacturers instructions; DAB chromogen.Primary antibody: dilution 1: 1000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

SRD5A2 Antibody Summary

• Immunogen: A synthetic peptide from amino acid region 190-250 of human SRD5A2 conjugated to blue carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SRD5A2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:2000
  • Immunohistochemistry-Paraffin 1:2000
  • Western Blot 1:4000
• Application Notes: The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen.

Reactivity Notes

Other species not yet tested but expected to work in rat.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for SRD5A2 Antibody

• 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
• 5 alpha-SR2
• EC 1.3.99.5
• MGC138457
• S5AR 2
• SR type 2
• SRD5A2
• Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
• steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
• Type II 5-alpha reductase

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRD5A2 Antibody (NBP1-46510)(3)

Publications using NBP1-46510

• Liu Z, Lin Z, Cao F et al. Upregulation of mir-1199-5p is associated with reduced type 2 5-alpha reductase expression in benign prostatic hyperplasia BMC urology 2022-11-07 [PMID: 36344974] (IHC-P, Human)
  
  Details:
  Dilution used in IHC 1:1500
• Liu Z, Lin Z, Cao F et al. Upregulation of miR-1199-5p is associated with reduced type 2 5-alpha reductase in benign prostatic hyperplasia Research Square 2022-03-23 [PMID: 36344974] (IF/IHC, Human)
• Lin ZM, Fan DD, Jin S et al. Methylated CpG dinucleotides in the 5-alpha reductase 2 gene may explain finasteride resistance in benign prostatic enlargement patients EBioMedicine 2020-11-23 [PMID: 33243958]

Bioinformatics

• Gene Symbol: SRD5A2
• Entrez:
  • 6716(Human)