SRD5A2 Antibody

Images

 
Western Blot: SRD5A2 Antibody [NBP1-46510] - WB on mouse tissue lysate. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:4000 incubated at 4C overnight.
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human pancreas. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more
Immunohistochemistry-Paraffin: SRD5A2 Antibody [NBP1-46510] - IHC-P on paraffin sections of human placenta. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using HRP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

SRD5A2 Antibody Summary

Immunogen
A synthetic peptide from the c-terminal region of human SRD5A2 (Steroid 5-alpha-reductase 2) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity
Specific for SRD5A2.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRD5A2
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 4000
  • Immunohistochemistry 1: 2000
  • Immunohistochemistry-Paraffin 1: 2000
Application Notes
Western Blot. A dilution of 1: 200 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin/Frozen.

Reactivity Notes

Human. Other species not yet tested but expected to work in rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability.

Alternate Names for SRD5A2 Antibody

  • 3-oxo-5-alpha-steroid 4-dehydrogenase 2,5 alpha-SR2
  • EC 1.3.99.5
  • MGC138457
  • S5AR 2
  • SR type 2
  • Steroid 5-alpha-reductase 2,3-oxo-5 alpha-steroid 4-dehydrogenase 2
  • steroid-5-alpha-reductase, alpha polypeptide 2 (3-oxo-5 alpha-steroid delta4-dehydrogenase alpha 2)
  • Type II 5-alpha reductase

Background

This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism, specifically pseudovaginal perineoscrotal hypospadias (PPSH).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56603
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
NBP2-38530
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-01151
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-1491
Species: Hu, Mu, Gp
Applications: WB, IHC, IHC-P, PEP-ELISA
NB100-1596
Species: Hu, Rt, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87217
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IF
H00005324-M02
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-32920
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13669
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-37502
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP1-32353
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF2656
Species: Hu
Applications: WB, IHC
NBP2-61764
Species: Hu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-31378
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-67150
Species: Hu, Mu, Rt
Applications: WB, ChIP, IHC, IHC-Fr, IHC-P, IP
NBL1-11228
Species: Hu
NBP1-59069
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for SRD5A2 Antibody (NBP1-46510) (0)

There are no publications for SRD5A2 Antibody (NBP1-46510).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SRD5A2 Antibody (NBP1-46510) (0)

There are no reviews for SRD5A2 Antibody (NBP1-46510). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SRD5A2 Antibody (NBP1-46510) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SRD5A2 Products

Bioinformatics Tool for SRD5A2 Antibody (NBP1-46510)

Discover related pathways, diseases and genes to SRD5A2 Antibody (NBP1-46510). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SRD5A2 Antibody (NBP1-46510)

Discover more about diseases related to SRD5A2 Antibody (NBP1-46510).
 

Pathways for SRD5A2 Antibody (NBP1-46510)

View related products by pathway.

PTMs for SRD5A2 Antibody (NBP1-46510)

Learn more about PTMs related to SRD5A2 Antibody (NBP1-46510).
 

Research Areas for SRD5A2 Antibody (NBP1-46510)

Find related products by research area.

Blogs on SRD5A2

There are no specific blogs for SRD5A2, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SRD5A2 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SRD5A2
Entrez