SRC1 Antibody (JB66-31)

Western Blot: SRC1 Antibody (JB66-31) [NBP2-75672] - Analysis of SRC1 on K562 cell lysate using anti- SRC1 antibody at 1:2000 dilution.

Immunohistochemistry-Paraffin: SRC1 Antibody (JB66-31) [NBP2-75672] - PFA fixed, paraffin-embedded human prostate cancer tissue sections. Heat-mediated antigen retreival was performed for 30 minutes in citrate buffer. IHC-P image submitted by a verified customer review.

Immunohistochemistry-Paraffin: SRC1 Antibody (JB66-31) [NBP2-75672] - Analysis of paraffin-embedded human lung cancer tissue using anti-SRC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SRC1 Antibody (JB66-31) [NBP2-75672] - Analysis of paraffin-embedded human thyroid gland tissue using anti-SRC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: SRC1 Antibody (JB66-31) [NBP2-75672] - Analysis of paraffin-embedded human breast tissue using anti- SRC1 antibody. Counter stained with hematoxylin.

Product Discontinued

SRC1 Antibody (JB66-31) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SRC1 aa 380-770 / 1,441. (SwissProt: Q15788 Human)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NCOA1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:500-1:2000
• Theoretical MW: 157 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SRC1 Antibody (JB66-31)

• bHLHe42
• bHLHe74
• Class E basic helix-loop-helix protein 74
• EC 2.3.1.48
• Hin-2 protein
• KAT13A
• MGC129719
• NCOA1
• NCoA-1
• nuclear receptor coactivator 1
• PAX3/NCOA1 fusion protein
• Protein Hin-2
• Renal carcinoma antigen NY-REN-52
• RIP160
• RIP160bHLHe74F-SRC-1
• SRC1
• SRC-1
• SRC1MGC129720
• Steroid receptor coactivator 1
• steroid receptor coactivator-1

Background

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC 1).SRC 1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 & H4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SRC1 Antibody (NBP2-75672) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 10/17/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Prostate cancer
• Species: Human
• Lot: JB66-31

Comments

• Comments: PFA fixed paraffin-embedded sections. Heat-mediated antigen retreival was performed for 30 minutes in citrate buffer.

Bioinformatics

• Gene Symbol: NCOA1