SR-BI/SR-BII Antibody

Images

 
Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.
Immunocytochemistry/ Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and ...read more
Flow Cytometry: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, B/N, IHC-Fr (-), KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SR-BI/SR-BII Antibody Summary

Immunogen
A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.
Localization
Lysosome; lysosomal membrane; multi-pass membrane protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 1:400
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Block/Neutralize
  • Knockout Validated
Application Notes
This SR-BI/SR-BII antibody is useful for Flow Cytometry (PMID: 22097902), Use in blocking/ neutralizing reported in scientific literature (PMID: 24859737). Immunocytochemistry, Western blot and Immunohistochemistry. In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands, and to as lesser extent testes, heart and mammary glands.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NB400-134 in the following applications:

Publications
Read Publications using
NB400-134 in the following applications:

  • BN
    1 publication
  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Rat (PMID: 22097902) and Human (PMID: 17105723) reactivity reported ins scientific literature

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for SR-BI/SR-BII Antibody

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1scavenger receptor class B type 1
  • CLA1CD36 antigen-like 1
  • CLA-1HDLQTL6
  • Collagen type I receptor, thrombospondin receptor-like 1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1scavenger receptor class B member 1
  • SR-BIMGC138242

Background

SR-BI (scavenger receptor class BI, SCARB1) is a member of the CD36 receptor family and acts as a major receptor for HDL for facilitating selective lipid uptake from lipoproteins in several tissues. The SR-BI gene gives rise to at least two mRNA splice variants (SR-BI and SR-BII), with the SR-BII variant being the main isoform in terms of mRNA levels in certain tissues, for example SR-BII mRNA is 48-fold higher in concentration than SR-BI in brain cells. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influences cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-52979
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB400-105
Species: Hu, Mu, Rt, Po, Ca, Ch, ChHa, Eq, Ha, Md, Pm, Rb
Applications: WB, ChIP, ELISA, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, Dual ISH-IHC, GS, KD, PCR
NBP2-38049
Species: Hu
Applications: IHC, IHC-P
NBP1-78159
Species: Hu, Rt, Po, Bv
Applications: WB, EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vitro, PLA, RIA
NB400-129
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NB300-558
Species: Hu, Bv, Rb
Applications: WB, ELISA, ICC/IF, IP
NB400-132
Species: Hu, Mu, Rt, ChHa, Ha, Mk, Rb
Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, In vitro, Flow-IC
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NBP2-75525
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NB100-65805
Species: Hu, Mu, Gt, Pm, Sh
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-00092
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PLA
NBP2-22106
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP

Publications for SR-BI/SR-BII Antibody (NB400-134)(8)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 5 applications: BN, FLOW, ICC/IF, IP, WB.


Filter By Application
BN
(1)
FLOW
(1)
ICC/IF
(1)
IP
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
Rat
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB400-134 Applications Species
Huang, L;Chambliss, KL;Gao, X;Yuhanna, IS;Behling-Kelly, E;Bergaya, S;Ahmed, M;Michaely, P;Luby-Phelps, K;Darehshouri, A;Xu, L;Fisher, EA;Ge, WP;Mineo, C;Shaul, PW; SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature Apr 24 2019 [PMID: 31019307] (WB, Human) WB Human
Kartz GA, Holme RL, Nicholson K, Sahoo, D. SR-BI/CD36 Chimeric Receptors Define Extracellular Subdomains of SR-BI Critical for Cholesterol Transport. Biochemistry 2014 Sep 23 [PMID: 25211142]
Emert B, Hasin-Brumshtein Y, Springstead JR et al. HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms [S] J Lipid Res. 2014 Jan 29 [PMID: 24859737] (BN, Human) BN Human
Hu J, Zhang Z, Shen W-J et al. Differential Roles of Cysteine Residues in Cellular Trafficking, Dimerization, and Function of the HDL Receptor, SR-BI. Biochemistry. 2011 Nov 18. [PMID: 22097902] (FLOW, Rat) FLOW Rat
Norgaard N, Holien T, Jonsson S et al. CpG-oligodeoxynucleotide inhibits Smad-dependent bone morphogenetic protein signaling: effects on myeloma cell apoptosis and in vitro osteoblastogenesis. J Immunol. 2010 [PMID: 20702733]
Schafer G, Guler R, Murray G et al. The role of scavenger receptor B1 in infection with Mycobacterium tuberculosis in a murine model. PLoS One 2009 [PMID: 20041149]
Harder CJ, Meng A, Rippstein P et al. SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells. J Biol Chem;282(2):1445-55. 2007 Jan 12. [PMID: 17105723] (ICC/IF, Human) ICC/IF Human
Silver, DL. A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver. J Biol Chem;277(37):34042-7. 2002 Sep 13. [PMID: 12119305] (IP, WB, Human) IP, WB Human

Review for SR-BI/SR-BII Antibody (NB400-134) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB400-134:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 08/07/2012
View

Summary

ApplicationWestern Blot
Sample Testedpurified SR-BI
SpeciesMouse
LotC-1

Blocking

Blocking Details5% low fat milk in PBS/Tween-20 (0.1%)

Primary Anitbody

Dilution RatioSR-BI Antibody (NB400-134) 1:1000

Secondary Antibody

Secondary DescriptionMouse anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare NA934V
Secondary Concentration1:10000 dilution

Details

Detection NotesChemiluminescense detection (ECL plus). 30 sec exposure. Positive control - KKB-1 antibody, neg - CD-36 protein

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SR-BI/SR-BII Antibody (NB400-134) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional SR-BI/SR-BII Products

Array NB400-134

Bioinformatics Tool for SR-BI/SR-BII Antibody (NB400-134)

Discover related pathways, diseases and genes to SR-BI/SR-BII Antibody (NB400-134). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SR-BI/SR-BII Antibody (NB400-134)

Discover more about diseases related to SR-BI/SR-BII Antibody (NB400-134).
 

Pathways for SR-BI/SR-BII Antibody (NB400-134)

View related products by pathway.

PTMs for SR-BI/SR-BII Antibody (NB400-134)

Learn more about PTMs related to SR-BI/SR-BII Antibody (NB400-134).
 

Research Areas for SR-BI/SR-BII Antibody (NB400-134)

Find related products by research area.

Blogs on SR-BI/SR-BII

There are no specific blogs for SR-BI/SR-BII, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Anonymous
08/07/2012
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SCARB1
Entrez
Uniprot
COVID-19 update