SR-BI/SR-BII Antibody - BSA Free

Western Blot: SR-BI/SR-BII Antibody [NB400-134] - Detection of RED-1 in 80 ug of total mouse liver lysates. Lane 1: wild-type mice, Lane 2: SR-BI deficient mice.

Immunocytochemistry/Immunofluorescence: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry-Paraffin: SR-BI/SR-BII Antibody [NB400-134] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using 5 ug/ml concentration of SR-BI/SR-BII antibody. Specific and expected granular membrane-cytoplasmic staining was observed in the hepatocytes. [40X Magnification]

Flow Cytometry: SR-BI/SR-BII Antibody [NB400-134] - SR-BI/SR-BII antibody was tested at 1:400 in NIH-3T3 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP, B/N, IHC-Fr (-), KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SR-BI/SR-BII Antibody - BSA Free Summary

• Immunogen: A peptide from the extracellular domain (residues 230-380) of Scavenger Receptor-BI/BII that was expressed as two tandem copies in bacteria using the pET system.
• Localization: Lysosome; lysosomal membrane; multi-pass membrane protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCARB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize reported in scientific literature (PMID 24859737)
  • Flow Cytometry 1:400. Use reported in scientific literature (PMID 22097902)
  • Immunocytochemistry/ Immunofluorescence 1:50-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Western Blot 1:1000
• Application Notes: In Western blot a band is observed at ~ 82 kDa in tissues that express SR-BI and/or SR-BII such as liver, ovary, adrenal glands, and to as lesser extent testes, heart and mammary glands.
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (PMID: 22097902) and Human (PMID: 17105723) reactivity reported ins scientific literature

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for SR-BI/SR-BII Antibody - BSA Free

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1scavenger receptor class B type 1
• CLA1CD36 antigen-like 1
• CLA-1HDLQTL6
• Collagen type I receptor, thrombospondin receptor-like 1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1scavenger receptor class B member 1
• SR-BIMGC138242

Background

SR-BI (scavenger receptor class BI, SCARB1) is a member of the CD36 receptor family and acts as a major receptor for HDL for facilitating selective lipid uptake from lipoproteins in several tissues. The SR-BI gene gives rise to at least two mRNA splice variants (SR-BI and SR-BII), with the SR-BII variant being the main isoform in terms of mRNA levels in certain tissues, for example SR-BII mRNA is 48-fold higher in concentration than SR-BI in brain cells. Similar differential SR-BII protein expression levels have also been detected in considerable amounts in other tissues, such as testes, retinal pigment epithelial cells, and spleen. SR-BII isoform differs from SR-BI only in its entirely different, yet highly conserved, cytoplasmic C-terminus which has been proposed to contains a signal responsible for marked alterations in cellular distribution as well as cellular trafficking of SR-BII compared with SR-BI. In contrast to SR-BI, SR-BII mediates the rapid uptake of significant amounts of HDL into endosomal recycling compartment by a pathway which is different from selective lipid uptake at the cell surface (mediated by SR-BI). Because SR-BII influences cellular cholesterol trafficking/homeostasis in a manner distinct from SR-BI, the relative expression as well as functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-BI/SR-BII Antibody (NB400-134)(11)

Publications using NB400-134

• Kakava S, Schlumpf E, Panteloglou G et al. Brain Endothelial Cells in Contrary to the Aortic Do Not Transport but Degrade Low-Density Lipoproteins via Both LDLR and ALK1 Cells 2022-09-28 [PMID: 36231005] (WB, Human)
• May SC, Dron JS, Hegele RA, Sahoo D Human Variant of Scavenger Receptor BI (R174C) Exhibits Impaired Cholesterol Transport Functions Journal of lipid research 2021-02-09 [PMID: 33577783]
• Xiao Wang, Huimin Wang, Bolin Xu, Dong Huang, Chao Nie, Longjun Pu, Gregory J M Zajac, Han Yan, Jingru Zhao, Fangyuan Shi, Brian T Emmer, Jia Lu, Rui Wang, Xiaohui Dong, Jianye Dai, Wenjing Zhou, Chu Wang, Ge Gao, Yan Wang, Cristen Willer, Xiangfeng Lu, Yuangang Zhu, Xiao-Wei Chen Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. Cell metabolism 2021-11-29 [PMID: 33186557]
• Huang, L;Chambliss, KL;Gao, X;Yuhanna, IS;Behling-Kelly, E;Bergaya, S;Ahmed, M;Michaely, P;Luby-Phelps, K;Darehshouri, A;Xu, L;Fisher, EA;Ge, WP;Mineo, C;Shaul, PW; SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis Nature 2019-04-24 [PMID: 31019307] (WB, Human)
• Kartz GA, Holme RL, Nicholson K, Sahoo, D. SR-BI/CD36 Chimeric Receptors Define Extracellular Subdomains of SR-BI Critical for Cholesterol Transport. Biochemistry 2014-09-23 [PMID: 25211142]
• Emert B, Hasin-Brumshtein Y, Springstead JR et al. HDL inhibits the effects of oxidized phospholipids on endothelial cell gene expression via multiple mechanisms [S] J Lipid Res. 2014-01-29 [PMID: 24859737] (BN, Human)
• Hu J, Zhang Z, Shen W-J et al. Differential Roles of Cysteine Residues in Cellular Trafficking, Dimerization, and Function of the HDL Receptor, SR-BI. Biochemistry. 2011-11-18 [PMID: 22097902] (FLOW, Rat)
• Norgaard N, Holien T, Jonsson S et al. CpG-oligodeoxynucleotide inhibits Smad-dependent bone morphogenetic protein signaling: effects on myeloma cell apoptosis and in vitro osteoblastogenesis. J Immunol. 2010-01-01 [PMID: 20702733]
• Schafer G, Guler R, Murray G et al. The role of scavenger receptor B1 in infection with Mycobacterium tuberculosis in a murine model. PLoS One 2009-01-01 [PMID: 20041149]
• Harder CJ, Meng A, Rippstein P et al. SR-BI undergoes cholesterol-stimulated transcytosis to the bile canaliculus in polarized WIF-B cells. J Biol Chem 2007-01-12 [PMID: 17105723] (ICC/IF, Human)
• Silver, DL. A carboxyl-terminal PDZ-interacting domain of scavenger receptor B, type I is essential for cell surface expression in liver. J Biol Chem;277(37):34042-7. 2002-09-13 [PMID: 12119305] (WB, IP, Human)

Review for SR-BI/SR-BII Antibody (NB400-134) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 08/07/2012

Summary

• Application: Western Blot
• Sample Tested: purified SR-BI
• Species: Mouse
• Lot: C-1

Bioinformatics

• Gene Symbol: SCARB1
• Entrez:
  • 20778(Mouse)
• Uniprot:
  • O35114()
  • Q61009(Mouse)