SR-BI Antibody (185-1G2 (B467)) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, B/N
• Clone: 185-1G2 (B467)
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 532

SR-BI Antibody (185-1G2 (B467)) [Alexa Fluor® 532] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunizing mice with stimulated human leukocytes.
• Specificity: This antibody is specific against SR-BI. The antibody recognises an epitope between amino acids 155 - 183. SR-BI is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. It acts as a receptor for hepatitis C virus in hepatocytes and appears to facilitate its cell entry as well as mediates uptake of M. fortuitum, E. coli and S. aureus.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SCARB1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SR-BI Antibody (185-1G2 (B467)) [Alexa Fluor® 532]

• CD36 and LIMPII analogous 1
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
• CD36 antigen
• CD36L1
• CD36L1scavenger receptor class B type 1
• CLA1 CD36 antigen-like 1
• CLA-1 HDLQTL6
• CLA1
• Collagen type I receptor, thrombospondin receptor-like 1
• HDLQTL6
• MGC138242
• SCARB1
• scavenger receptor class B type III
• scavenger receptor class B, member 1
• SRB1 scavenger receptor class B member 1
• SR-B1
• SRBI
• SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2). At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport. References 1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375 2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373 3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCARB1