SR-AI/MSR Antibody (268318) [Fluorescein]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse SR-AI/MSR Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1797F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 268318
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

SR-AI/MSR Antibody (268318) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SR‑AI/MSR
  Trp79-Ser454
  Accession # AAA39747
• Specificity: Detects mouse SR‑AI/MSR in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SR-AI is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: MSR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SR-AI/MSR Antibody (268318) [Fluorescein]

• CD204 antigen
• CD204
• Macrophage acetylated LDL receptor I and II
• macrophage scavenger receptor 1
• macrophage scavenger receptor type III
• MSR1
• phSR1
• phSR2
• SCARA1
• SCARA1macrophage scavenger receptor types I and II
• Scavenger receptor class A member 1
• scavenger receptor class A, member 1
• SR-A
• SRAI
• SR-AI

Background

The Scavenger Receptor (SR) family comprises a group of functionally defined membrane receptors that share the common ability to bind and internalize modified forms of Low Density Lipoproteins (mLDL) (1‑3). Family members are classified alphabetically. The A class includes four proteins: the three subtypes of SR-A (AI, AII, and AIII) that are generated by alternative splicing of the same gene, and a structurally similar protein named MARCO (4). All A class SRs are multidomain, trimeric type II trans-membrane proteins. SR-AI has an N-terminal cytoplasmic domain, a transmembrane domain, a spacer domain, an alpha -helical coiled coil, a collagen-like domain and a C-terminal cysteine-rich domain. SR-A is expressed by most tissue macrophages, dendritic cells and Kupffer cells. It is also highly expressed by microglia in neonatal as well as Alzheimer’ Disease brains. SR-AI binds a broad range of polyanionic ligands including modified proteins (e.g. oxidized, acetylated or maleylated LDL, advanced glycation end-product proteins), polyribonucleotides (polyguanosine and polyinosine), polysaccharides (dextran sulfate, fucoidan), phospholipids (phosphatidylserine), bacterial products (lipopolysaccharide and lipoteichoic acid) and selected chemical compounds (silica, crocidolite asbestos). The ligand-binding region has been localized to a positively charged region in the carboxyl end of the collagen-like domain. Based on its ligand binding characteristics, SR-AI is implicated in many physiological and pathophysiological functions. Studies using SR-A knockout mouse have also suggested roles of SR-A in atherogenesis, host defense and innate immunity, acquired immune responses, macrophage adhesion, and phagocytosis of apoptotic cells (1‑3). Over aa 83-458, mouse and human SR-AI share 71% amino acid sequence identity.

1. Daugherty, A. et al. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs 2:223.
2. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
3. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
4. Elomaa, O. et al. (1995) Cell 80:603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SR-AI/MSR Antibody (FAB1797F)(3)

Publications using FAB1797F

• Lee, MG;Song, Y;Kang, H; Exploring the complex immunomodulatory effects and gut defense via oral administration of Astragali radix water extract to normal mice BMC Complementary Medicine and Therapies 2024-01-01 [PMID: 39375623] (Flow Cytometry, Mouse)
• Daisuke Fujioka, Yosuke Watanabe, Takamitsu Nakamura, Takashi Yokoyama, Keiji Miyazawa, Makoto Murakami et al. Group V Secretory Phospholipase A₂ Regulates Endocytosis of Acetylated LDL by Transcriptional Activation of PGK1 in RAW264.7 Macrophage Cell Line Journal of Atherosclerosis and Thrombosis 2022-05-01 [PMID: 33775979] (Flow Cytometry, Mouse)
• TK Kwak, HS Jang, MG Lee, YS Jung, DO Kim, YB Kim, JI Kim, H Kang Effect of Orally Administered Atractylodes macrocephala Koidz Water Extract on Macrophage and T Cell Inflammatory Response in Mice Evid Based Complement Alternat Med, 2018-08-07;2018(0):4041873. 2018-08-07 [PMID: 30174703] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: MSR1
• Uniprot:
  • AAA39747()