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Immunocytochemistry/ Immunofluorescence: SQRDL Antibody [NBP1-84510] - Staining of human cell line A549 shows localization to mitochondria. Antibody staining is shown in green.
Analysis in mouse cell line NIH-3T3.
Orthogonal Strategies: Analysis in human cell lines A-549 and HEK293 using Anti-SQRDL antibody. Corresponding SQRDL RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Orthogonal Strategies: Analysis in human colon and placenta tissues. Corresponding RNA-seq data are presented for the same tissues.
Staining of human lymphoid tissues shows strong positivity in cytoplasm granular in non-germinal center cells.
Staining of human placenta shows very weak granular cytoplasm positvity in trophoblastic cells.
Staining of human prostate shows strong granular cytoplasm positivity in glandular cells.
Staining of human rectum shows strong granular cytoplasm positivity in glandular cells.
Staining of human colon shows strong granular cytoplasm positivity in glandular cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: TSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWG
Predicted Species
Rat (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SQOR
Purity
Affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified
Alternate Names for SQRDL Antibody - BSA Free
CGI-44
sulfide dehydrogenase like
sulfide quinone reductase-like (yeast)
sulfide:quinone oxidoreductase, mitochondrial
Background
SQRDL catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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