SPRY2 Antibody - BSA Free

Immunoprecipitation: SPRY2 Antibody [NBP2-04100] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Sprouty 2 antibody NBP2-04099 used for IP at 6 ug/mg lysate. Sprouty 2 was also immunoprecipitated by rabbit anti-Sprouty 2 antibody NBP2-04100, which recognizes a downstream epitope. For blotting immunoprecipitated Sprouty 2, NBP2-04100 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SPRY2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 265 and 315 of human Sprouty 2 using the numbering given in entry NP_005833.1 (GeneID 10253).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPRY2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-10 mcg/mg

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SPRY2 Antibody - BSA Free

• hSPRY2
• MGC23039
• protein sprouty homolog 2
• sprouty (Drosophila) homolog 2
• sprouty 2
• sprouty homolog 2 (Drosophila)
• SPRY2
• spry-2

Background

Sprouty 2 encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPRY2
• Uniprot:
  • NP_005833.1(Human)