FRS2 Antibody (462910)

Western blot shows lysates of MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 462910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FRS2 Antibody (462910) Summary

• Immunogen: E. coli-derived recombinant human FRS2
  Asn121-Asn449
  Accession # Q8WU20
• Specificity: Detects human, mouse, and rat FRS2 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FRS2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FRS2 Antibody (462910)

• FGFR signalling adaptor
• FGFR-signaling adaptor SNT
• fibroblast growth factor receptor substrate 2
• FRS2
• FRS2A
• FRS2alpha
• SNT
• SNT1
• SNT-1FGFR substrate 2
• Suc1-associated neurotrophic factor target 1

Background

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FRS2 Antibody (MAB4069)(14)

Publications using MAB4069

• O'Loughlin, E;Zhang, Y;Chiasson-MacKenzie, C;Dave, P;Rheinbay, E;Stott, S;McClatchey, AI; Distinct phenotypic consequences of cholangiocarcinoma-associated FGFR2 alterations depend on biliary epithelial maturity bioRxiv : the preprint server for biology 2024-09-02 [PMID: 39282270] (Western Blot, Mouse)
• Zechen Wang, Viswanathan Muthusamy, Daniel P. Petrylak, Karen S. Anderson Tackling FGFR3-driven bladder cancer with a promising synergistic FGFR/HDAC targeted therapy npj Precision Oncology 7/21/2023 [PMID: 37479885]
• Subbiah, V;Sahai, V;Maglic, D;Bruderek, K;Toure, BB;Zhao, S;Valverde, R;O'Hearn, PJ;Moustakas, DT;Schonherr, H;Gerami-Moayed, N;Taylor, AM;Hudson, BM;Houde, DJ;Pal, D;Foster, L;Gunaydin, H;Ayaz, P;Sharon, DA;Goyal, L;Schram, AM;Kamath, S;Sherwin, CA;Schmidt-Kittler, O;Jen, KY;Ricard, F;Wolf, BB;Shaw, DE;Bergstrom, DA;Watters, J;Casaletto, JB; RLY-4008, the first highly selective FGFR2 inhibitor with activity across FGFR2 alterations and resistance mutations Cancer discovery 2023-06-04 [PMID: 37270847] (Western Blot, Human)
• HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer British Journal of Cancer, 2022-07-01;0(0):. 2022-07-01 [PMID: 35778553] (Western Blot, Human)
• Renuka Raman, Jacques A. Villefranc, Timothy M. Ullmann, Jessica Thiesmeyer, Viviana Anelli, Jun Yao et al. Inhibition of FGF receptor blocks adaptive resistance to RET inhibition in CCDC6-RET–rearranged thyroid cancer Journal of Experimental Medicine 6/6/2022 [PMID: 35510953]
• Donghong Liu, Wenbin Liu, Xi Chen, Jianhua Yin, Longteng Ma, Mei Liu et al. circKCNN2 suppresses the recurrence of hepatocellular carcinoma at least partially via regulating miR‐520c‐3p/methyl‐DNA‐binding domain protein 2 axis Clinical and Translational Medicine 1/20/2022 [PMID: 35051313]
• Hong Xiang, Abigael G. Chan, Ago Ahene, David I. Bellovin, Rong Deng, Amy W. Hsu et al. Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer mAbs 11/1/2021 [PMID: 34719330]
• S Wu, T Ou, N Xing, J Lu, S Wan, C Wang, X Zhang, F Yang, Y Huang, Z Cai Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer Nat Commun, 2019-02-12;10(1):720. 2019-02-12 [PMID: 30755618] (Western Blot, Human)
• E Terzuoli, F Corti, G Nannelli, A Giachetti, S Donnini, M Ziche Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1 Int J Mol Sci, 2018-09-06;19(9):. 2018-09-06 [PMID: 30200598] (Western Blot, Human)
• L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth Cell Death Dis, 2018-02-15;9(3):262. 2018-02-15 [PMID: 29449529] (Western Blot, Human)
• Joseph M. Rutkowski, Johanne Pastor, Kai Sun, S. Claire Park, I. Alexandru Bobulescu, Christopher T. Chen et al. Adiponectin alters renal calcium and phosphate excretion through regulation of klotho expression Kidney International 2017-02-01 [PMID: 27914707] (Western Blot, Mouse, Transgenic Mouse)
• David J. Elzi, Meihua Song, Barron Blackman, Susan T. Weintraub, Dolores López-Terrada, Yidong Chen et al. FGF19 functions as autocrine growth factor for hepatoblastoma Genes & Cancer 7/7/2016 [PMID: 27382436]
• Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease. Kidney Int, 2014-01-08;85(6):1340-50. 2014-01-08 [PMID: 24402093] (Western Blot, Rat)
• Bryant MR, Marta CB, Kim FS, Bansal R Phosphorylation and lipid raft association of fibroblast growth factor receptor-2 in oligodendrocytes. Glia, 2009-07-01;57(9):935-46. 2009-07-01 [PMID: 19053057] (Western Blot, Human)

Review for FRS2 Antibody (MAB4069) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19735729
• Species: Mouse

Bioinformatics

• Gene Symbol: FRS2
• Uniprot:
  • Q8WU20()