FRS2 Antibody (462910)

Western blot shows lysates of MCF‑7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat FRS2 Monoclonal Antibody (Catalog # MAB4069) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FRS2 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 462910
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

FRS2 Antibody (462910) Summary

• Immunogen: E. coli-derived recombinant human FRS2
  Asn121-Asn449
  Accession # Q8WU20
• Specificity: Detects human, mouse, and rat FRS2 in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: FRS2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FRS2 Antibody (462910)

• FGFR signalling adaptor
• FGFR-signaling adaptor SNT
• fibroblast growth factor receptor substrate 2
• FRS2
• FRS2A
• FRS2alpha
• SNT
• SNT1
• SNT-1FGFR substrate 2
• Suc1-associated neurotrophic factor target 1

Background

FRS2 (FGF R substrate 2; also known as SNT and FRS2 alpha ) is a 70‑90 kDa member of the FRS family of lipid-anchored docking proteins. It is an intermediary between FGF R and other RTK receptors and their Ras/MAPK signaling cascades. FRS2 contains a membrane-anchoring myristoylation signal (aa 1‑6), a PTB domain (aa 13‑115) that interacts with FGF and NGF receptors, and a C-terminal Tyr-rich region (aa 196‑471) that serves as a docking site for GRB‑2 and SHP‑2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FRS2 Antibody (MAB4069)(6)

Publications using MAB4069

• HA Nicholson, L Sawers, RG Clarke, KJ Hiom, MJ Ferguson, G Smith Fibroblast growth factor signalling influences homologous recombination-mediated DNA damage repair to promote drug resistance in ovarian cancer British Journal of Cancer, 2022-07-01;0(0):. 2022-07-01 [PMID: 35778553] (Western Blot, Human)
• S Wu, T Ou, N Xing, J Lu, S Wan, C Wang, X Zhang, F Yang, Y Huang, Z Cai Whole-genome sequencing identifies ADGRG6 enhancer mutations and FRS2 duplications as angiogenesis-related drivers in bladder cancer Nat Commun, 2019-02-12;10(1):720. 2019-02-12 [PMID: 30755618] (Western Blot, Human)
• E Terzuoli, F Corti, G Nannelli, A Giachetti, S Donnini, M Ziche Bradykinin B2 Receptor Contributes to Inflammatory Responses in Human Endothelial Cells by the Transactivation of the Fibroblast Growth Factor Receptor FGFR-1 Int J Mol Sci, 2018-09-06;19(9):. 2018-09-06 [PMID: 30200598] (Western Blot, Human)
• L Xu, X Meng, N Xu, W Fu, H Tan, L Zhang, Q Zhou, J Qian, S Tu, X Li, Y Lao, H Xu Gambogenic acid inhibits fibroblast growth factor receptor signaling pathway in erlotinib-resistant non-small-cell lung cancer and suppresses patient-derived xenograft growth Cell Death Dis, 2018-02-15;9(3):262. 2018-02-15 [PMID: 29449529] (Western Blot, Human)
• Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease. Kidney Int, 2014-01-08;85(6):1340-50. 2014-01-08 [PMID: 24402093] (Western Blot, Rat)
• Bryant MR, Marta CB, Kim FS, Bansal R Phosphorylation and lipid raft association of fibroblast growth factor receptor-2 in oligodendrocytes. Glia, 2009-07-01;57(9):935-46. 2009-07-01 [PMID: 19053057] (Western Blot, Human)

Review for FRS2 Antibody (MAB4069) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: cynthia morimoto WB Mouse 02/20/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 19735729
• Species: Mouse

Bioinformatics

• Gene Symbol: FRS2
• Uniprot:
  • Q8WU20()