Spi-B Antibody [DyLight 680]

• Reactivity: Mu
• Applications: CyTOF-ready, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: DyLight 680

Spi-B Antibody [DyLight 680] Summary

• Immunogen: E. coli-derived recombinant mouse Spi-B
  Tyr18-Glu167 (Tyr110Phe)
  Accession # O35906
• Specificity: Detects mouse Spi-B in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human Spi-B is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SPIB
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry
  • Intracellular Staining by Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Spi-B Antibody [DyLight 680]

• Spi-B transcription factor (Spi-1/PU.1 related)
• SpiB
• Spi-B
• transcription factor Spi-B

Background

SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPIB