SPG3A Antibody

Western Blot: SPG3A Antibody [NBP2-93512] - Analysis of extracts of various cell lines, using SPG3A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 180s.

Immunocytochemistry/Immunofluorescence: SPG3A Antibody [NBP2-93512] - Analysis of A549 cells using ATL1 antibody. Blue: DAPI for nuclear staining

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SPG3A Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATL1 (NP_056999.2).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SPG3A Antibody

• AD-FSP
• ATL1
• atlastin GTPase 1
• atlastin1
• atlastin-1
• Brain-specific GTP-binding protein
• EC 3.6.5.-
• FSP1
• GBP3
• GBP-3
• GTP-binding protein 3
• Guanine nucleotide-binding protein 3
• guanylate-binding protein 3
• hGBP3
• Spastic paraplegia 3 protein A
• spastic paraplegia 3A (autosomal dominant)
• SPG3
• SPG3A
• SPG3AhGBP3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATL1