GBP1 Antibody

Western Blot: GBP1 Antibody [NBP2-03972] - Analysis of GBP1 using GBP1 polyclonal antibody. Human HCT-116 lysate in the absence (1) and presence (2) of immunizing peptide and mouse RAW cell lysate (3) probed with GBP1 antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: GBP1 Antibody [NBP2-03972] - Analysis of GBP1 using GBP1 polyclonal antibody. Human rectum tissue probed with GBP1 antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

GBP1 Antibody Summary

• Description: Novus Biologicals Rabbit GBP1 Antibody (NBP2-03972) is a polyclonal antibody validated for use in IHC and WB. Anti-GBP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 500-550 of human GBP1 was used as the immunogen for this antibody.
• Predicted Species: Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GBP1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for GBP1 Antibody

• GBP-1
• GTP-binding protein 1
• Guanine nucleotide-binding protein 1
• guanylate binding protein 1, interferon-inducible
• guanylate binding protein 1, interferon-inducible, 67kDa
• huGBP-1
• interferon-induced guanylate-binding protein 1

Background

GBP1 or guanlyate binding protein 1 is a 68 kDa GTPase and member of the GTPase1 family of molecules (Kunzelman, 2006). GBP1 expression is induced by Interferon g as well as other inflammatory cytokines including IL-1a, IL-1b and TNF-a (Naschberger, 2004). Induction of GBP1 expression by poly I:C, a prototypical TLR3 ligand, as well as GBP1 expression dependency on NF-kB and IFNa response elements implicate TLR pathway involvement (Naschberger, 2004). Inversely proportional expression of GBP1 and MMP-1 as well as other related molecules to adhesion and migration show a clear functional role of GBP1 in suppressing angiogenesis and migration especially of endothelial cells (Guenzi, 2003). As a regulatory molecule it is centrally involved in signal transduction of inflammatory and cell migration networks.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GBP1 Antibody (NBP2-03972)(1)

Publication using NBP2-03972

• Huang H, Even Z, Wang Z et al. Proteomics Profiling Reveals Regulation of Immune Response to Salmonella enterica Serovar Typhimurium Infection in Mice Infection and immunity 2022-12-13 [PMID: 36511704]

Bioinformatics

• Gene Symbol: GBP1
• Uniprot:
  • NP_002044.2(Human)