Speedy/Ringo Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Speedy/Ringo Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to a C-terminal region of human Speedy/Ringo (within residues 200-286). This immunogen is conserved in both isoforms 1 and 2. [Swiss-Prot Q5MJ69]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPDYA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Speedy/Ringo antibody is useful for Western blot analysis, where a band is seen at ~36 kDa (isoform 2). After a longer exposure, a 31 kDa band can be detected (isoform 1). There is also a non-specific band at ~58 kDa. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 19187565).
Readout System

Reactivity Notes

Sheep reactivity reported in scientific literature (PMID: 19187565). Human reactivity reported in scientific literature (PMID: 24037419). Rat reactivity reported in scientific literature (PMID: 19075091).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Speedy/Ringo Antibody [Biotin]

  • hSpy/Ringo A
  • MGC110856
  • Rapid inducer of G2/M progression in oocytes A
  • RINGO A
  • RINGO3
  • SPDY1
  • speedy homolog 1 (Drosophila)
  • speedy homolog A (Xenopus laevis)
  • speedy protein A
  • speedy-1
  • Spy1
  • SPY1MGC57218

Background

Speedy/Ringo (Spy1) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. It mediates cell survival during the DNA damage process through activation of CDK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Speedy/Ringo Antibody (NB100-2521B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-2521AF350
Alexa Fluor 405 NB100-2521AF405
Alexa Fluor 488 NB100-2521AF488
Alexa Fluor 532 NB100-2521AF532
Alexa Fluor 594 NB100-2521AF594
Alexa Fluor 647 NB100-2521AF647
Alexa Fluor 700 NB100-2521AF700
Alexa Fluor 750 NB100-2521AF750
Biotin NB100-2521B
DyLight 350 NB100-2521UV
DyLight 405 NB100-2521V
DyLight 488 NB100-2521G
DyLight 550 NB100-2521R
DyLight 594 NB100-2521DL594
DyLight 650 NB100-2521C
DyLight 680 NB100-2521FR
DyLight 755 NB100-2521IR
FITC NB100-2521F
HRP NB100-2521H
Janelia Fluor 549 NB100-2521JF549
Janelia Fluor 646 NB100-2521JF646

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Bioinformatics Tool for Speedy/Ringo Antibody (NB100-2521B)

Discover related pathways, diseases and genes to Speedy/Ringo Antibody (NB100-2521B). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Speedy/Ringo Antibody (NB100-2521B)

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PTMs for Speedy/Ringo Antibody (NB100-2521B)

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Research Areas for Speedy/Ringo Antibody (NB100-2521B)

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Blogs on Speedy/Ringo

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Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

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Bioinformatics

Gene Symbol SPDYA