Speedy/Ringo Antibody

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Western Blot: Speedy/Ringo Antibody [NB100-2521] - Detection of Speedy/Ringo in mouse testis (2ug/ml).

Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Speedy/Ringo Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal region of human Speedy/Ringo (within residues 200-286). This immunogen is conserved in both isoforms 1 and 2. [Swiss-Prot Q5MJ69]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPDYA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
This Speedy/Ringo antibody is useful for Western blot analysis, where a band is seen at ~36 kDa (isoform 2). After a longer exposure, a 31 kDa band can be detected (isoform 1). There is also a non-specific band at ~58 kDa. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 19187565).
Positive Control
Testis Lysate (NB820-59672)
Control Peptide
Speedy/Ringo Peptide (NB100-2521PEP)
Publications
Read Publications using
NB100-2521 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    1 publication

Reactivity Notes

Mouse. Sheep reactivity reported in scientific literature (PMID: 19187565). Predicted to react with human based on 100% sequence homology. Human reactivity reported in scientific literature (PMID: 24037419). Rat reactivity reported in scientific literature (PMID: 19075091)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.1% Sodium Azide
Concentration
1.1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Speedy/Ringo Antibody

  • hSpy/Ringo A
  • MGC110856
  • Rapid inducer of G2/M progression in oocytes A
  • RINGO A
  • RINGO3
  • SPDY1
  • speedy homolog 1 (Drosophila)
  • speedy homolog A (Xenopus laevis)
  • speedy protein A
  • speedy-1
  • Spy1
  • SPY1MGC57218

Background

Speedy/Ringo (Spy1) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. It mediates cell survival during the DNA damage process through activation of CDK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Speedy/Ringo Antibody (NB100-2521)(6)

We have publications tested in 3 confirmed species: Human, Rat, Sheep.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Rat
(1)
Sheep
(1)
All Species

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Product General Protocols

View specific protocols for Speedy/Ringo Antibody (NB100-2521): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Speedy/Ringo Antibody (NB100-2521) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2521AF405
Alexa Fluor 488 Labeled NB100-2521AF488
Alexa Fluor 647 Labeled NB100-2521AF647
Alexa Fluor 700 Labeled NB100-2521AF700
Biotin Labeled NB100-2521B
DyLight 405 Labeled NB100-2521V
DyLight 488 Labeled NB100-2521G
DyLight 550 Labeled NB100-2521R
DyLight 650 Labeled NB100-2521C
FITC Labeled NB100-2521F
HRP Labeled NB100-2521H

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Bioinformatics Tool for Speedy/Ringo Antibody (NB100-2521)

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Bioinformatics

Gene Symbol SPDYA