Speedy/Ringo Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, ShSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit

Speedy/Ringo Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a C-terminal region of human Speedy/Ringo (within residues 200-286). This immunogen is conserved in both isoforms 1 and 2. [Swiss-Prot Q5MJ69]
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Gene
SPDYA
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
FITC
Format
FITC (A=495, E=519)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
Application Notes
This Speedy/Ringo antibody is useful for Western blot analysis, where a band is seen at ~36 kDa (isoform 2). After a longer exposure, a 31 kDa band can be detected (isoform 1). There is also a non-specific band at ~58 kDa. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 19187565).

Reactivity Notes

Mouse. Sheep reactivity reported in scientific literature (PMID: 19187565). Predicted to react with human based on 100% sequence homology. Human reactivity reported in scientific literature (PMID: 24037419). Rat reactivity reported in scientific literature (PMID: 19075091)

Alternate Names for Speedy/Ringo Antibody [FITC]

  • hSpy/Ringo A
  • MGC110856
  • Rapid inducer of G2/M progression in oocytes A
  • RINGO A
  • RINGO3
  • SPDY1
  • speedy homolog 1 (Drosophila)
  • speedy homolog A (Xenopus laevis)
  • speedy protein A
  • speedy-1
  • Spy1
  • SPY1MGC57218

Background

Speedy/Ringo (Spy1) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. It mediates cell survival during the DNA damage process through activation of CDK2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol SPDYA

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