Spectrin beta 3 Antibody [DyLight 405]

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: DyLight 405

Spectrin beta 3 Antibody [DyLight 405] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: The epitope recognized by this antibody maps to a region between residues 2150 and 2200 of Spectrin beta 3 using the numbering given in entry NP_008877.1
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPTBN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
• Application Notes: This Spectrin beta 3 antibody is useful for Immunoprecipitation, Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections. It is not recommended for Western blot analysis.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
  
  Recommended applications are based on validated applications from the unconjugated base product NB110-58783. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Spectrin beta 3 Antibody [DyLight 405]

• beta-III Spectrin
• glutamate transporter EAAT4-associated protein 41
• GTRAP41
• KIAA0302
• SCA5
• SCAR14
• Spectrin beta 3
• spectrin beta chain, brain 2
• Spectrin beta III
• spectrin, beta, non-erythrocytic 2
• Spectrin, non-erythroid beta chain 2
• spinocerebellar ataxia 5
• SPTBN2

Background

Spectrin beta 3 (SPTBN2) belongs to spectrins family of proteins which are essential to mechanical support/structural membrane integrity maintenance in erythrocytes, stabilizing cell-cell contacts, and localizing ion channels as well as cell adhesion molecules within specific subdomains of plasma membranes. Vertebrate spectrins have two alpha-subunits (alpha-I/alpha-II), four beta-subunits (beta-I-beta-IV) and a beta-H subunit creating diversity and specialization of function. Spectrin beta 3 is primarily expressed in nervous tissues with highest expression levels in the cerebellum, where it is found in Purkinje cell soma and dendrites. Spectrin beta 3 interacts with EAAT4, the glutamate transporter predominately expressed in Purkinje cells, and stabilizes it at the plasma membrane leading to glutamate clearance from the synaptic cleft, and resulting in both modulation of glutamatergic neurotransmission as well as prevention of glutamate-mediated neurotoxicity. Defective spectrin beta 3 has been established as causing factor in spinocerebellar ataxia type 5 (SCA5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPTBN2