Spectrin beta 1 Antibody (4C3)

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Western Blot: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of 25 ug of Hela cell lysates.
Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of A431 cells (right) compared to a negative control without ...read more
Immunohistochemistry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Neu5Gc co-staining with beta spectrin, CD11b, CD8 or Pax7 in normal, BMD or DMD human muscle. Otherwise normal, Becker muscular dystrophy (BMD) and ...read more
Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Hela cells compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of Spectrin beta-1 (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without ...read more
Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the membrane of Human skeletal muscle (right) compared with a negative control in the absence of primary ...read more
Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm of Human prostate carcinoma (right) compared with a negative control in the absence of ...read more
Immunohistochemistry-Paraffin: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis showing positive staining in the cytoplasm and membrane of Mouse skeletal muscle (right) compared with a negative control in the ...read more
Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of SH-SY5Y cells compared to an isotype control (blue).
Flow Cytometry: Spectrin beta 1 Antibody (4C3) [NB300-574] - Analysis of NIH/3T3 cells compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
4C3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Spectrin beta 1 Antibody (4C3) Summary

Immunogen
Purified human erythrocyte beta-1 spectrin.
Localization
Cytoplasmic
Specificity
Detects spectrin from erythrocytes, brain and muscle cells. This has been shown to specifically detect the two known alternatively spliced forms of spectrin, beta-1 epsilon-1, present in erythrocytes, and beta-1 epsilon-2, present in nerve and striated muscle cells. It does not cross-react with alpha-2 spectrin or either of the fodrin subunits.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPTB
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100
  • Flow Cytometry 0.5 - 2 ug
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:200
  • Immunohistochemistry 1:20 - 1:200
  • Immunohistochemistry-Frozen 1:20 - 1:200
  • Immunohistochemistry-Paraffin 1:20 - 1:200
Application Notes
WB: Detects an approx. 246 kDa protein representing beta-1 spectrin in rat skeletal muscle homogenate. IF: Staining of beta-1 spectrin in rat skeletal muscle fibers results in intense staining of the sarcolemma. Antigen integrity can be compromised if aldehyde fixatives are left in contact with the protein for extended periods of time. If paraformaldehyde is used as a fixative, exposure should be limited to 5 minutes or less of no more than a 2% solution. IHC usage was reported in scientitic literature (PMID: 9142435). IHC-Fr usage was reported in scientific literature (PMID: 24505439).
Publications
Read Publications using
NB300-574 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 24505439). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Spectrin beta 1 Antibody (4C3)

  • beta-I spectrin
  • EL3
  • erythrocyte
  • HS2
  • HSPTB1
  • membrane cytoskeletal protein
  • spectrin, beta, erythrocytic
  • SPTB1

Background

Spectrin (Sp), the most abundant of the erythrocyte membrane skeleton proteins, helps these cells maintain their characteristic biconcave shape while remaining flexible and elastic. Erythrocyte Sp is a heterodimer composed of a 280 kDa alpha subunit and a 246 kDa beta subunit which associate in a side-to-side, antiparallel configuration to form a 100 nm rod-like structure. Sp in other tissues may be composed of distinct but homologous alpha and beta subunits, sometimes referred to as fodrin. A newly introduced nomenclature designates the Sp subunits of the erythrocyte as alpha-1 and beta-1, and the fodrin subunits as alpha-2 and beta-2. Alternatively spliced forms of each are designated as epsilon-1, epsilon-2, etc. (e.g. beta-1 epsilon-1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Spectrin beta 1 Antibody (NB300-574)(2)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 2 applications: IHC-Fr, WB.


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IHC-Fr
(1)
WB
(1)
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(1)
Canine
(1)
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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SPTB
Entrez