SPARC Antibody (1B2)

Western Blot: SPARC Antibody (1B2) [H00006678-M02] - SPARC monoclonal antibody (M02), clone 1B2. Analysis of SPARC expression in human kidney.

Western Blot: SPARC Antibody (1B2) [H00006678-M02] - SPARC monoclonal antibody (M02), clone 1B2 Analysis of SPARC expression in A-431.

Western Blot: SPARC Antibody (1B2) [H00006678-M02] - Analysis of SPARC expression in transfected 293T cell line by SPARC monoclonal antibody (M02), clone 1B2.Lane 1: SPARC transfected lysate(34.6 KDa).Lane 2: Non-transfected lysate.

ELISA: SPARC Antibody (1B2) [H00006678-M02] - Detection limit for recombinant GST tagged SPARC is approximately 3ng/ml as a capture antibody.

Product Discontinued

SPARC Antibody (1B2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SPARC (AAH04974.1, 1 a.a. ~ 303 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI
• Specificity: SPARC - secreted protein, acidic, cysteine-rich (osteonectin) (1B2)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPARC
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: IHC-P usage reported in scientific literature (PMID: 27544129).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPARC Antibody (1B2)

• Basement-membrane protein 40
• BM-40
• ONcysteine-rich protein
• Osteonectin
• Secreted protein acidic and rich in cysteine
• secreted protein, acidic, cysteine-rich (osteonectin)
• SPARC

Background

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SPARC Antibody (H00006678-M02)(2)

Publications using H00006678-M02

• Davis EJ, Zhao L, Lucas DR et al. SPARC expression in patients with high-risk localized soft tissue sarcoma treated on a randomized phase II trial of neo/adjuvant chemotherapy. BMC Cancer. 2016-08-22 [PMID: 27544129] (IHC-P)
• Piconese S, Costanza M, Tripodo C et al. The matricellular protein SPARC supports follicular dendritic cell networking toward Th17 responses. J Autoimmun. 2011-09-28 [PMID: 21962567]

Bioinformatics

• Gene Symbol: SPARC