SP1 Antibody (S01-9G6)

Western Blot: SP1 Antibody (S01-9G6) [NBP3-14976] - Western blot detection of SP1 in K562, 3T3, Hela cell lysates using SP1 Rabbit mAb (1:1000 diluted). Predicted band size: 81kDa. Observed band size: 90kDa.

Immunohistochemistry-Paraffin: SP1 Antibody (S01-9G6) [NBP3-14976] - Immunohistochemistry of SP1 in paraffin-embedded Human colon cancer tissue using SP1 Rabbit mAb at dilution 1/50

• Reactivity: Hu
• Applications: WB, IHC
• Clone: S01-9G6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SP1 Antibody (S01-9G6) Summary

• Description: Novus Biologicals Rabbit SP1 Antibody (S01-9G6) (NBP3-14976) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human SP1 (Uniprot#: P08047)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/1000
• Theoretical MW: 90 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for SP1 Antibody (S01-9G6)

• Sp1 transcription factor
• specificity protein 1
• transcription factor Sp1
• TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP1