SP1 Antibody (5I3A8)

Western Blot: SP1 Antibody (5I3A8) [NBP3-15837] - Western blot analysis of extracts of various cell lines, using SP1 antibody (NBP3-15837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunocytochemistry/Immunofluorescence: SP1 Antibody (5I3A8) [NBP3-15837] - Immunofluorescence analysis of NIH-3T3 cells using SP1 antibody (NBP3-15837). Blue: DAPI for nuclear staining.

Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using SP1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 across chromosome 14 (upper panel) and the genomic region encompassing TINF2, a representative gene enriched in SP1 (lower panel).

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Chromatin Immunoprecipitation: SP1 Antibody (5I3A8) [SP1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from 293T cells using 5 ug of SP1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of SP1 in the representative genomic region surrounding TINF2 gene.

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: SP1 Antibody (5I3A8) [SP1] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using SP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: SP1 Antibody (5I3A8) [SP1] - Confocal imaging of C6 cells using SP1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, ChIP
• Clone: 5I3A8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SP1 Antibody (5I3A8) Summary

• Description: Novus Biologicals Rabbit SP1 Antibody (5I3A8) (NBP3-15837) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human SP1 (P08047). TTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQLGLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SP1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) 5μg antibody for 10μg-15μg of Chromatin
  • ELISA Recommended starting concentration is 1 μg/mL
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin 1:200 - 1:2000
  • Western Blot 1:1000 - 1:2000
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SP1 Antibody (5I3A8)

• Sp1 transcription factor
• specificity protein 1
• transcription factor Sp1
• TSFP1

Background

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SP1