SP-D Antibody (1.7) - BSA Free

Immunohistochemistry-Paraffin: SP-D Antibody (1.7) [NBP1-41050] - Staining of frozen pig lung with Mouse anti pig Surfactant Protein D

• Reactivity: Po
• Applications: WB, IHC
• Clone: 1.7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SP-D Antibody (1.7) - BSA Free Summary

• Description: Novus Biologicals Mouse SP-D Antibody (1.7) - BSA Free (NBP1-41050) is a monoclonal antibody validated for use in IHC and WB. Anti-SP-D Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified porcine surfactant protein D
• Specificity: This antibody detects porcine surfactant protein D (pSP-D), a 138kD member of the collectin family of proteins. Surfactant protein D is thought to play a significant role in the innate immune response against microorganisms within the lungs and at other mucosal surfaces. SP-D is a multifunctional protein which binds selectively to saccharides and lipids on microbial surfaces promoting antimicrobial responses and regulating surfactant lipid homeostasis. SP-D expression is induced in lung surfactant in response to aerogenous bacterial infection, and upon binding to bacteria, promotes phagocytosis and antigen presentation by dendritic cells. Recent studies suggest that pSP-D could serve as a serum biomarker specific for bronchopneumonia in pigs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SFTPD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; TRIS/EDTA buffer pH8.0 is recommended for this purpose.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for SP-D Antibody (1.7) - BSA Free

• COLEC7collectin-7
• Collectin 7
• Collectin-7
• Lung surfactant protein D
• PSPD
• PSP-D
• SFTP4
• SFTP4pulmonary surfactant-associated protein D
• SFTPD
• SPD
• SP-D
• SP-Dpulmonary surfactant apoprotein
• surfactant protein D
• surfactant, pulmonary-associated protein D
• surfactant-associated protein, pulmonary 4

Background

Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SP-D Antibody (NBP1-41050)(3)

Publications using NBP1-41050

• Mayor RS, Finch KE, Zehr J et al. Maternal high-fat diet is associated with impaired fetal lung development. Am J Physiol Lung Cell Mol Physiol 2015-08-15 [PMID: 26092997] (Mouse)
• Soerensen, CM et al. Pulmonary infections in swine induce altered porcine surfactant protein D expression and localization to dendritic cells in bronchial-associated lymphoid tissue. Immunology. Immunology. [PMID: 16011521]
• Soerensen, CM et al. Purification, characterization and immunolocalization of porcine surfactant protein D. Immunology. 114: 72-82. 2005-01-01 [PMID: 15606797]

Bioinformatics

• Gene Symbol: SFTPD
• Entrez:
  • 397198(Porcine)
• Uniprot:
  • Q9N1X4(Porcine)