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HAF017). A specific band was detected for SP-D at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody." class="big_lightbox" target="_blank">
MAB19201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SP-D Monoclonal Antibody (Catalog # MAB1920) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular head regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is 88 ng/ml in healthy individuals (2).
![Western Blot SP-D Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/SPD_AF1920_Western_Blot_17882.jpg)
![ELISA SP-D Antibody (292201) [Unconjugated]](http://images.novusbio.com/images/antibody/SPD_MAB1920_ELISA_23579.jpg)
![Sandwich ELISA SP-D Antibody Pair [HRP]](http://images.novusbio.com/fullsize/SP-D-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79547-img0001.jpg)
![N/A SP-D [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_SPD_MSFPD0_ELISA_1031.jpg)
![N/A SP-D [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_SPD_DY683905_ELISA_2498.jpg)
![Simple Western SP-D Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/SPD_AF1920_Simple_Western_18698.jpg)
![N/A SP-D [HRP]](http://images.novusbio.com/fullsize/elisa/SPD_MSFPD0_ELISA_487.jpg)
