SOX10 Antibody (1E6)

Immunohistochemistry-Paraffin: SOX10 Antibody (1E6) [H00006663-M01] - Analysis of monoclonal antibody to SOX10 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1.5 ug/ml.

Product Discontinued

SOX10 Antibody (1E6) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SOX10 (NP_008872, 336 a.a. ~ 433 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KPPGVALPTVSPPGVDAKAQVKTETAGPQGPPHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSGQASGLYSAFSYMGPSQR
• Specificity: SOX10 - SRY (sex determining region Y)-box 10 (1E6)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOX10
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26024302). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SOX10 Antibody (1E6)

• DOM
• MGC15649
• PCWH
• SOX10
• SOX-10
• SRY (sex determining region Y)-box 10
• SRY-related HMG-box gene 10
• transcription factor SOX-10
• WS2E
• WS4
• WS4C
• WS4mouse, human homolog of

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOX10 Antibody (H00006663-M01)(5)

Publications using H00006663-M01

• Areias J, Sola C, Chastagnier Y et al. Whole-brain characterization of apoptosis after sevoflurane anesthesia reveals neuronal cell death patterns in the mouse neonatal neocortex Scientific reports 2023-09-07 [PMID: 37679476] (IHC, Mouse)
  
  Details:
  Dilution: 1:1000
• McCoy CR, Stadelman BS, Brumaghim JL et al. Arsenic and Its Methylated Metabolites Inhibit the Differentiation of Neural Plate Border Specifier Cells Chem. Res. Toxicol. 2015-06-10 [PMID: 26024302] (IHC-P, Mouse)
  
  Details:
  SOX10 antibody (clone 1E6) was used for IHC-P staining of embryoid bodies which were generated / differentiated from mouse P19 mouse embryonal carcinoma cell line using the hanging drop method (Wang and Yang 2008, J Visualized Exp. 17, 825). The staining assay implicated antigen rertieval via citric acid buffer pH 6 using microwave, 1 hour blocking with 1× PBS, 5%BSA, and 0.05% Tween-20, use of primary at 1:200 dilution with 4C ON incubation, detection with Alexa Fluor conjugated secondary antibody (Figure 2, Figure 4).
• Kim JE, Chang SE, Yeo UC et al. Histopathological study of the treatment of melasma lesions using a low-fluence Q-switched 1064-nm neodymium:yttrium-aluminium-garnet laser. Clin Exp Dermatol. 2013-03-01 [PMID: 23397944]
• Llamas-Velasco M, Mentzel T, Requena L et al. Cutaneous PEComa does not harbor TFE3 gene fusions. Immunohistochemical and molecular study of 17 cases. Histopathology 2013-03-26
• Mehta A, Davey J, Gharpuray-Pandit D et al. Cutaneous Myoepithelial Neoplasms on Acral Sites Show Distinctive and Reproducible Histopathologic and Immunohistochemical Features The American journal of surgical pathology 2022-03-31 [PMID: 35354162]

Bioinformatics

• Gene Symbol: SOX10
• Entrez:
  • 6663(Human)
• Uniprot:
  • NP_008872(Human)