SorCS2 Antibody [Unconjugated]

Western blot shows lysates of human kidney (medulla) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human SorCS2 Antigen Affinity-purified Antibody (Catalog # AF4238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SorCS2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SorCS2 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Sheep Anti-Human SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4238) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and their processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SorCS2 Antibody [Unconjugated] Summary

• Immunogen: NS0-derived recombinant human SorCS2
  Ser70-Gly1078
  Accession # Q96PQ0
• Specificity: Detects human SorCS2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) SorCS2 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS1 and rhSorCS3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SORCS2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS2 Antibody [Unconjugated]

• KIAA1329VPS10 domain receptor protein
• SorCS2
• sortilin-related VPS10 domain containing receptor 2
• VPS10 domain-containing receptor SorCS2

Background

SorCS2 is a type I transmembrane glycoprotein receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). Family receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in other locations (1‑3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well‑defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4‑6). Human SorCS2 is synthesized as a 1159 amino acid (aa) prepro form with a 50 aa signal sequence and a potential furin-type proteolytic processing site at aa 119. This would produce a mature SorCS2 protein of 1040 aa with a 959 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, human SorCS2 shares 89%, 88%, 88% and 79% aa identity with mouse, rat, equine and canine SorCS2, respectively. It also shares 46% aa identity with the ECD of both SorCS1 and SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
2. Rezgaoui, M. et al. (2001) Mech. Dev. 100:335.
3. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
4. Hermey, G. et al. (2006) Biochem. J. 395:285.
5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
6. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SorCS2 Antibody (AF4238)(4)

Publications using AF4238

• Gospodinova KO, Olsen D, Kaas M et al. Loss of SORCS2 is Associated with Neuronal DNA Double-Strand Breaks Cellular and Molecular Neurobiology 2023-01-01 [PMID: 34741697]
• Gospodinova KO, Olsen D, Kaas M et al. Loss of SORCS2 is Associated with Neuronal DNA Double-Strand Breaks Cellular and Molecular Neurobiology 2023-01-01 [PMID: 34741697]
• Beatrice Vignoli, Gabriele Sansevero, Manju Sasi, Roberto Rimondini, Robert Blum, Valerio Bonaldo et al. Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention Communications Biology 2021-10-05 [PMID: 34611268]
• Q Ma, J Yang, TA Milner, JG Vonsattel, ME Palko, L Tessarollo, BL Hempstead SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease JCI Insight, 2017-05-04;2(9):. 2017-05-04 [PMID: 28469074]

Bioinformatics

• Gene Symbol: SORCS2
• Uniprot:
  • Q96PQ0()