SorCS3 Antibody (339624)

Altered subcellular localization of TrkB in primary neurons and brains from S1/3 KO miceAWorkflow of the cell surface proteome analysis in primary neurons.BResults of quantitative label‐free proteomics comparing the surface proteomes of WT and S1/3 KO primary cortical neurons. Plot represents –log10(P‐value) and log2 (relative levels S1/3KO/WT) obtained for each protein. For proteins that were detected in either S1/3 KO or WT samples only, the log2(S1/3 KO/WT) was set to 10 or ‐10, respectively. Threshold values are 1.3 for –log10(P‐value) and ±0.3 for log2(S1/3 KO/WT). Proteins showing higher (green) or lower (red) surface levels in S1/3 KO as compared to WT neurons are indicated. Proteins with unchanged cell surface levels are indicated in blue. n = 3 biological replicates/group (with each biological replicate run in two technical replicates).CScheme of brain membrane fractionation used to analyze subcellular localization of TrkB in vivo (see method section for details).DRepresentative Western blotting of TrkB in various brain membrane fractions obtained as described in panel (C). Highlighted lanes represent P3 and P2 fractions, quantified in (E). Detection of PSD95, synaptophysin, and Golgin 97 was used to assess the accuracy of subcellular fractionation.EQuantification of total TrkB distribution between P3 (light membrane fraction) and P2 (synaptosomal fraction) from Western blots exemplified in panel (D) (n = 5 mice/group, 7–12 weeks of age). The TrkB pool is shifted toward the light membrane (P3) fraction in S1/3 KO brains. Data are shown as mean ± SEM and analyzed using two‐way ANOVA with Bonferroni post‐test. ****P < 0.0001.F, GCo‐immunoprecipitation of SORCS1 and SORCS3 with TrkB. TrkB was immunoprecipitated from transiently transfected Chinese hamster ovary cells and the presence of SORCS1 or SORCS3 in the immunoprecipitates documented by Western blotting. Panel input shows levels of the indicated proteins in the cell lysates prior to immunoprecipitation. Panel IP documents co‐immunoprecipitation of SORCS1 (F) and SORCS3 (G) from cells expressing (lanes 3) but not from cells lacking TrkB (lanes 2). The experiment was replicated twice.Source data are available online for this figure. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29440124), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 339624
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

SorCS3 Antibody (339624) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SorCS3
  Ala131-Ser1122
  Accession # Q8VI51
• Specificity: Detects human and mouse SorCS3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SorCS1 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SORCS3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS3 Antibody (339624)

• 6330404A12Rik
• KIAA1059SORCS
• SorCS3
• sortilin-related VPS10 domain containing receptor 3
• VPS10 domain receptor protein SORCS 3 (SORCS3)
• VPS10 domain-containing receptor SorCS3

Background

SorCS3 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein‑sorting 10 protein) family of receptors that includes sortilin, SorLA, and three SorCS proteins (1, 2). It is synthesized as an ~1220 amino acid (aa) preproform with a 33 aa signal sequence and a 100 aa propeptide. After proteolytic release of the propeptide at a furin‑type consensus sequence, the mature SorCS3 is a 100‑110 kDa transmembrane protein with a 992 aa extracellular/lumenal domain (ECD). Mouse and human SorCS3 ECD share 92% aa sequence identity. They also share ~70% and ~45% aa identity with SorCS1 and SorCS2 ECDs, respectively. The ECD contains an imperfect leucine‑rich repeat (LRR) and a Vps10p domain that binds both pro- and mature NGF (2, 3). The metalloproteinase TACE/ADAM17 is able to cleave SorCS3 near the membrane either constitutively, or at an increased rate when induced by phorbol esters (4). The shed ECD is able to bind PDGF‑BB and the NGF propeptide (4). Unlike sortilin, the SorCS3 propeptide has no known function; it does not block NGF binding or propeptide cleavage (3, 5). SorCS3 is predominantly expressed on the plasma membrane (3). It can be slowly internalized but, despite the presence of a sorting domain, there is no evidence for SorCS3‑mediated intracellular trafficking activity (3). It is expressed in the embryonic and adult central nervous system in areas distinct from that of SorCS1 and SorCS2 (1). Neuronal activity upregulates SorCS3 expression in the hippocampus (1).

1. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
2. Hampe, W. et al. (2001) Hum. Genet. 108:529.
3. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
4. Hermey, G. et al. (2006) Biochem. J. 395:285.
5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SorCS3 Antibody (MAB3067)(4)

Publications using MAB3067

• A Subkhangul, AR Malik, G Hermey, O Popp, G Dittmar, T Rathjen, MN Poy, A Stumpf, PS Beed, D Schmitz, T Breiderhof, TE Willnow SORCS1 and SORCS3 control energy balance and orexigenic peptide production EMBO Rep., 2018-02-12;19(4):. 2018-02-12 [PMID: 29440124] (Western Blot, Mouse)
• K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi Prions amplify through degradation of the VPS10P sorting receptor sortilin PLoS Pathog., 2017-06-30;13(6):e1006470. 2017-06-30 [PMID: 28665987] (Western Blot, Mouse)
• Moore K, Bryant ZJ, Ghatnekar G et al. A synthetic connexin 43 mimetic peptide augments corneal wound healing. Exp Eye Res. [PMID: 23876491]
  
  Details:
  Citation using the Unpurified form of this antibody.
• Toby A. Ferguson, Steven S. Scherer Neuronal cadherin (NCAD) increases sensory neurite formation and outgrowth on astrocytes Neuroscience Letters 8/1/2012 [PMID: 22698587]

Bioinformatics

• Gene Symbol: SORCS3
• Uniprot:
  • Q8VI51()