Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Biotinylated rrNLGN-1v2 immobilized on a streptavidin-coated plate at 2 µg/mL
(100 μL/well) can bind rhNeurexin 3 beta /Fc Chimera with an apparent KD <40 nM.
Source
Mouse myeloma cell line, NS0-derived human Neurexin 3 beta/NRXN3b protein
Human Neurexin 3 beta
(Ser35 - Thr357)
Accession # NP_620426
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ser35
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NRXN3
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
61.1 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-96 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF

  • Neurexin 3 beta

Background

The alpha and beta forms of Neurexins 1 - 3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3 - 5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta. It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta. Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6 - 9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is upregulated after short term exposure of mice to cocaine (11 - 13).

  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
  3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
  4. Rowen, L. et al. (2002) Genomics 79:587.
  5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.
  6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.
  7. Ullrich, B. et al. (1995) Neuron 14:497.
  8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  9. Ichtchenko, K. et al. (1995) Cell 81:435.
  10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.
  11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.
  12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.
  13. Kelai, S. et al. (2008) Neuroreport 19:751.

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Bioinformatics

Gene Symbol NRXN3
Entrez
Uniprot