Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Biotinylated rrNLGN-1v2 immobilized on a streptavidin-coated plate at 2 µg/mL
  (100 μL/well) can bind rhNeurexin 3 beta /Fc Chimera with an apparent K_D <40 nM.
• Source: Mouse myeloma cell line, NS0-derived human Neurexin 3 beta/NRXN3b protein
  

  Human Neurexin 3 beta (Ser35 - Thr357) Accession # NP_620426	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_620426
• N-terminal Sequence: Ser35
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: NRXN3
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 61.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-96 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neurexin 3 beta Fc Chimera Protein, CF

• Neurexin 3 beta

Background

The alpha and beta forms of Neurexins 1 - 3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1 - 3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3 - 5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta . It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta . Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6 - 9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is upregulated after short term exposure of mice to cocaine (11 - 13).

1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
4. Rowen, L. et al. (2002) Genomics 79:587.
5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.
6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.
7. Ullrich, B. et al. (1995) Neuron 14:497.
8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
9. Ichtchenko, K. et al. (1995) Cell 81:435.
10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.
11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.
12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.
13. Kelai, S. et al. (2008) Neuroreport 19:751.

Bioinformatics

• Gene Symbol: NRXN3
• Uniprot:
  • NP_620426(Human)