SorCS2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

SorCS2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: NS0-derived recombinant human SorCS2
  Ser70-Gly1078
  Accession # Q96PQ0
• Specificity: Detects human SorCS2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse (rm) SorCS2 is observed and less than 1% cross-reactivity with recombinant human (rh)&nbs
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS2 Antibody [Alexa Fluor® 488]

• KIAA1329VPS10 domain receptor protein
• SorCS2
• sortilin-related VPS10 domain containing receptor 2
• VPS10 domain-containing receptor SorCS2

Background

SorCS2 is a type I transmembrane glycoprotein receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). Family receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in other locations (1‑3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well‑defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4‑6). Human SorCS2 is synthesized as a 1159 amino acid (aa) prepro form with a 50 aa signal sequence and a potential furin-type proteolytic processing site at aa 119. This would produce a mature SorCS2 protein of 1040 aa with a 959 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, human SorCS2 shares 89%, 88%, 88% and 79% aa identity with mouse, rat, equine and canine SorCS2, respectively. It also shares 46% aa identity with the ECD of both SorCS1 and SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.