Reactivity | HuSpecies Glossary |
Applications | WB, ICC/IF |
Clonality | Polyclonal |
Host | Goat |
Conjugate | Unconjugated |
Concentration | LYOPH |
Immunogen | Mouse myeloma cell line NS0-derived recombinant human SorCS1 Ser111-Ser1099 (Ser231Gly) Accession # Q8WY21.3 |
Specificity | Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed. |
Source | N/A |
Isotype | IgG |
Clonality | Polyclonal |
Host | Goat |
Gene | SORCS1 |
Purity | Immunogen affinity purified |
Purity Statement | Antigen Affinity-purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | Immunogen affinity purified |
Reconstitution Instructions | Reconstitute at 0.2 mg/mL in sterile PBS. |
SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).
Images | Ratings | Applications | Species | Date | Details | ||||||||||||
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reviewed by:
Anonymous |
IP | Human | 12/18/2014 |
Summary
Blocking
Details
Comments
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reviewed by:
Anonymous |
ELISA | Human | 12/18/2014 |
Summary
Blocking
Details
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reviewed by:
Anonymous |
IF | Other | 12/18/2014 |
Summary
Blocking
Details
Comments
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Secondary Antibodies |
Isotype Controls |
Diseases for SorCS1 Antibody (AF3457)Discover more about diseases related to SorCS1 Antibody (AF3457).
| Pathways for SorCS1 Antibody (AF3457)View related products by pathway.
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PTMs for SorCS1 Antibody (AF3457)Learn more about PTMs related to SorCS1 Antibody (AF3457).
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Anonymous 12/18/2014 |
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Application: | IP | |
Species: | Human |
Anonymous 12/18/2014 |
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Application: | ELISA | |
Species: | Human |
Anonymous 12/18/2014 |
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Application: | IF | |
Species: | Other |