SorCS1 Antibody

Images

 
Immunoprecipitation: SorCS1 Antibody [AF3457] - IP of human SorCS1 transfected into HEK293 cells. Lane 4 is lysate of untransfected cells. Image courtesy of pAbmAbs.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SorCS1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human SorCS1
Ser111-Ser1099 (Ser231Gly)
Accession # Q8WY21.3
Specificity
Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SORCS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 3 Reviews rated 5
using
AF3457 in the following applications:

Publications
Read Publications using
AF3457 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS1 Antibody

  • FLJ41758
  • FLJ43475
  • FLJ44957
  • hSorCS
  • SORCS receptor 1
  • SORCS
  • SorCS1
  • sortilin-related VPS10 domain containing receptor 1
  • VPS10 domain receptor protein SORCS 1
  • VPS10 domain receptor SorCS
  • VPS10 domain-containing receptor SorCS1

Background

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
  3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
  4. Clee, S.M. et al. (2006) Nat. Genet. 38:688.
  5. Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
  6. Hermey, G. et al. (2006) Biochem. J. 395:285.
  7. Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
  8. Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SorCS1 Antibody (AF3457)(2)

Reviews for SorCS1 Antibody (AF3457) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Other.

Reviews using AF3457:
Filter by Applications
IP
(1)
ELISA
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunoprecipitation SorCS1 AF3457
Enlarge
reviewed by:
Anonymous
IP Human 12/18/2014
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Summary

ApplicationImmunoprecipitation
Sample TestedIP of human SorCS1 transfected into HEK293 cells
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
ELISA Human 12/18/2014
View

Summary

ApplicationELISA
Sample TestedN/A
SpeciesHuman

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
 
reviewed by:
Anonymous
IF Other 12/18/2014
View

Summary

ApplicationImmunofluorescence
Sample TestedCHO-cells stable expressing mouse SorCS1 variant c
SpeciesOther

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SorCS1 Antibody (AF3457) (0)

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Secondary Antibodies

 

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Diseases for SorCS1 Antibody (AF3457)

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Recent Reviews

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5
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2
0
1
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Anonymous
12/18/2014
Application: IP
Species: Human

 
Anonymous
12/18/2014
Application: ELISA
Species: Human

 
Anonymous
12/18/2014
Application: IF
Species: Other

Bioinformatics

Gene Symbol SORCS1
Entrez
Uniprot