SorCS1 Antibody

MPEP decreases SORT1 expression and increases extracellular PGRN in mammalian cell lines. (A and B) M17 cells were treated with control siRNA (siR-Ctrl) or gene-specific SORT1 siRNA (siR-SORT1). (A) Intracellular levels of PGRN, SORT1 and GAPDH were evaluated by western blot at a 48 h time-point. (B) Suppression of SORT1 levels increased extracellular PGRN levels. (C) Chemical name and structure of MPEP. (D–I) Treatment of M17 cells (D and E), HeLa cells (F and G) or NIH3T3 cells (H and I) with MPEP for 24 h dose dependently reduced SORT1 levels (D, F and H) and increased exPGRN levels (E, G and I) at 10 and 20 μM. (J) Under the same conditions, MPEP did not affect levels of SORLA, SORCS1 and ubiquitinated proteins in M17 cells. ***P < 0.001 versus vehicle control, analysis performed by one-way ANOVA followed by Tukey's post-test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24163244), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SorCS1 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SorCS1
  Ser111-Ser1099 (Ser231Gly)
  Accession # Q8WY21.3
• Specificity: Detects human SorCS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse SorCS1 is observed and less than 1% cross-reactivity with recombinant human (rh) SorCS2 and rhSorCS3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SORCS1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS1 Antibody

• FLJ41758
• FLJ43475
• FLJ44957
• hSorCS
• SORCS receptor 1
• SORCS
• SorCS1
• sortilin-related VPS10 domain containing receptor 1
• VPS10 domain receptor protein SORCS 1
• VPS10 domain receptor SorCS
• VPS10 domain-containing receptor SorCS1

Background

SorCS1 is a type I transmembrane receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. Three splicing variants (SorCS1a, b and c) differ only in their cytoplasmic domains (3). All variants are predominantly expressed in the central nervous system, but SorCS1 can also be identified in heart, kidney and pancreatic islets (2‑5). SorCS1a mediates endocytosis, and only ~10% of it is expressed on the cell surface. SorCS1b shows higher surface expression (~45%) and is much less involved in endocytosis. SorCS1c is intermediate. Human SorCS1a is synthesized as a 1159 amino acid (aa) preproform with a 33 aa signal sequence and a 77 aa propeptide. After proteolytic processing at a furin-type consensus sequence, the mature SorCS1a is a 1049 aa, 130 kDa protein with a 989 aa extracellular/lumenal domain (ECD). Within the ECD, human SorCS1 shares 93%, 94%, 93% and 98% aa identity with mouse, rat, bovine and canine SorCS1, respectively. It also shares 70% and 46% aa identity with the ECD of human SorCS3 and SorCS2, respectively. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain and binds the growth factor PDGF-BB (1, 2, 6). Expression in the hippocampus indicates that SorCS1 may modulate PDGF-BB activity in this location (6). SorCS1 has also been identified as a susceptibility gene for type 2 diabetes in overweight females (4). Consequently, it has been proposed to affect insulin secretion by modifying PDGF-mediated growth of the islet vasculature (7). The 80 kDa ECD may be constitutively or inducibly shed, mainly via the metalloproteinase TACE/ADAM17 (6). The shed soluble form also binds PDGF. The cellular portion appears to undergo regulated intramembrane proteolysis (8).

1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
2. Hermey, G. et al. (1999) Biochem. Biophys. Res. Commun. 266:347.
3. Hermey, G. et al. (2003) J. Biol. Chem. 278:7390.
4. Clee, S.M. et al. (2006) Nat. Genet. 38:688.
5. Hermey, G. et al. (2001) Neurosci. Lett. 313:83.
6. Hermey, G. et al. (2006) Biochem. J. 395:285.
7. Goodarzi, M.O. et al. (2007) Diabetes Apr 10 [Epub ahead of print].
8. Nyborg, A.C. et al. (2006) Mol. Neurodegen. 1:3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SorCS1 Antibody (AF3457)(3)

Publications using AF3457

• K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi Prions amplify through degradation of the VPS10P sorting receptor sortilin PLoS Pathog., 2017-06-30;13(6):e1006470. 2017-06-30 [PMID: 28665987] (Western Blot, Mouse)
• Lee WC, Almeida S, Prudencio M et al. Targeted manipulation of the sortilin-progranulin axis rescues progranulin haploinsufficiency. Hum Mol Genet. 11/7/2013 [PMID: 24163244]
• Reitz C, Tokuhiro S, Clark LN, Conrad C, Vonsattel JP, Hazrati LN, Palotas A, Lantigua R, Medrano M, Z Jimenez-Velazquez I, Vardarajan B, Simkin I, Haines JL, Pericak-Vance MA, Farrer LA, Lee JH, Rogaeva E, George-Hyslop PS, Mayeux R SORCS1 alters amyloid precursor protein processing and variants may increase Alzheimer&#039;s disease risk. Ann. Neurol., 2011-01-01;69(1):47-64. 2011-01-01 [PMID: 21280075] (ICC, Western Blot, Human)

Reviews for SorCS1 Antibody (AF3457) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Other.

reviewed by: Verified Customer IP Human 12/18/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: IP of human SorCS1 transfected into HEK293 cells
• Species: Human

reviewed by: Verified Customer ELISA Human 12/18/2014

Summary

• Application: ELISA
• Sample Tested: N/A
• Species: Human

reviewed by: Verified Customer IF Other 12/18/2014

Summary

• Application: Immunofluorescence
• Sample Tested: CHO-cells stable expressing mouse SorCS1 variant c
• Species: Other

Bioinformatics

• Gene Symbol: SORCS1
• Uniprot:
  • Q8WY21.3()