SorCS2 Antibody [Unconjugated]

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SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more
SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SorCS2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SorCS2
Ala118-Gly1078
Accession # Q9EPR5
Specificity
Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SORCS2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Reviewed Applications
Read 5 Reviews rated 4.4
using
AF4237 in the following applications:

Publications
Read Publications using
AF4237 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS2 Antibody [Unconjugated]

  • KIAA1329VPS10 domain receptor protein
  • SorCS2
  • sortilin-related VPS10 domain containing receptor 2
  • VPS10 domain-containing receptor SorCS2

Background

Mouse SorCS2 is a 110‑130 kDa member of the Vps10p (vacuolar protein sorting 10 protein)-D family of sorting receptors. It is synthesized as a 1159 amino acid (aa) preproprecursor that undergoes proteolytic processing to generate a 1042 aa mature type I transmembrane receptor. The extracellular domain shows an imperfect LRR and a Vps10p domain. SorCS2 is plasma membrane expressed and may undergo proteolytic cleavage. Over aa 118-1078, mouse SorCS2 is 88% and 98% aa identical to human and rat SorCs2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SorCS2 Antibody (AF4237)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
IHC-Fr
(2)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(4)
All Species

Reviews for SorCS2 Antibody (AF4237) (5) 4.45

Average Rating: 4.4
(Based on 5 reviews)
We have 5 reviews tested in 3 species: Mouse, Rat, Other.

Reviews using AF4237:
Filter by Applications
WB
(3)
IP
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(3)
Rat
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Mouse 12/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedHippocampal lysates from p20 mice
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  5
reviewed by:
Anonymous
WB Rat 12/17/2014
View

Summary

ApplicationWestern Blot
Sample TestedPrimary rat Schwann cells
SpeciesRat

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsFive-star review on pAbmAbs http://pabmabs.com/
  3
reviewed by:
Anonymous
IP Mouse 12/17/2014
View

Summary

ApplicationImmunoprecipitation
Sample TestedIP of endogenous SorCS2 in mouse tissues as well as SorCS2 knockout mouse
SpeciesMouse

Blocking

Blocking Detailsn/a

Details

Detection Notesn/a

Comments

CommentsThree-star review on pAbmAbs http://pabmabs.com/
  4
reviewed by:
Anonymous
ICC Mouse 12/16/2014
View

Summary

ApplicationImmunocytochemistry
Sample TestedMouse Tissues
SpeciesMouse
LotN/A

Blocking

Blocking DetailsICC: 2 ug/ml

Details

Detection NotesWestern blot on CNS and PNS tissue homogenates and in primary neuron cultures.

Comments

CommentsCells or sections from SorCS2 knockout mouse are blank confirming the specificity of the signal.Five-star review on pAbmAbs http://pabmabs.com/
  5
reviewed by:
Anonymous
WB Other 12/16/2014
View

Summary

ApplicationWestern Blot
Sample Testedmouse tissue homogenates
SpeciesOther
LotN/A

Blocking

Blocking Details0.2 ug/ml

Details

Detection NotesWestern blot on CNS and PNS tissue homogenates and in primary neuron cultures.

Comments

CommentsWe use it for detecting and IP of endogenous SorCS2 in mouse tissues. Cells or sections from SorCS2 knockout mouse are blank confirming the specificity of the signal.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SorCS2 Antibody (AF4237) (0)

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Secondary Antibodies

 

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Blogs on SorCS2

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Recent Reviews

4.4
5
3
4
1
3
1
2
0
1
0

 
Anonymous
12/17/2014
Application: WB
Species: Mouse

 
Anonymous
12/17/2014
Application: WB
Species: Rat

 
Anonymous
12/17/2014
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol SORCS2
Uniprot