SorCS2 Antibody [Unconjugated]

SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

SorCS2 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse SorCS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4237) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SorCS2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SorCS2
  Ala118-Gly1078
  Accession # Q9EPR5
• Specificity: Detects mouse SorCS2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SorCS2 and less than 5% cross-reactivity with rhSorCS1 and recombinant mouse SorCS1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SORCS2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SorCS2 Antibody [Unconjugated]

• KIAA1329VPS10 domain receptor protein
• SorCS2
• sortilin-related VPS10 domain containing receptor 2
• VPS10 domain-containing receptor SorCS2

Background

Mouse SorCS2 is a 110‑130 kDa member of the Vps10p (vacuolar protein sorting 10 protein)-D family of sorting receptors. It is synthesized as a 1159 amino acid (aa) preproprecursor that undergoes proteolytic processing to generate a 1042 aa mature type I transmembrane receptor. The extracellular domain shows an imperfect LRR and a Vps10p domain. SorCS2 is plasma membrane expressed and may undergo proteolytic cleavage. Over aa 118-1078, mouse SorCS2 is 88% and 98% aa identical to human and rat SorCs2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SorCS2 Antibody (AF4237)(11)

Publications using AF4237

• Ayon-Olivas, M;Wolf, D;Andreska, T;Granado, N;Lüningschrör, P;Ip, CW;Moratalla, R;Sendtner, M; Dopaminergic Input Regulates the Sensitivity of Indirect Pathway Striatal Spiny Neurons to Brain-Derived Neurotrophic Factor Biology 2023-10-23 [PMID: 37887070] (Immunocytochemistry, Mouse)
• Skeldal, S;Voss, LF;Lende, J;Pedersen, SB;Mølgaard, S;Ollendorff, MK;Demange, P;Bentsen, AH;Fuglsang, M;Sander, MR;Buttenschøn, H;Gustafsen, C;Madsen, P;Glerup, S; Alternative splicing regulates adaptor protein binding, trafficking, and activity of the Vps10p domain receptor SorCS2 in neuronal development The Journal of biological chemistry 2023-07-26 [PMID: 37507021] (Western Blot, N/A)
• Y Li, X Guan, Y He, X Jia, L Pan, Y Wang, Y Han, R Zhao, J Yang, T Hou ProBDNF signaling is involved in periodontitis-induced depression-like behavior in mouse hippocampus International immunopharmacology, 2023-02-02;116(0):109767. 2023-02-02 [PMID: 36738676] (Immunoprecipitation, Western Blot, Transgenic Mouse)
• J Yang, Q Ma, I Dincheva, J Giza, D Jing, T Marinic, TA Milner, A Rajadhyaks, FS Lee, BL Hempstead SorCS2 is required for social memory and trafficking of the NMDA receptor Mol. Psychiatry, 2020-01-27;0(0):. 2020-01-27 [PMID: 31988435] (IHC, Mouse)
• AR Malik, K Szydlowska, K Nizinska, A Asaro, EA van Vliet, O Popp, G Dittmar, R Fritsche-G, JA Kirwan, A Nykjaer, K Lukasiuk, E Aronica, TE Willnow SorCS2 Controls Functional Expression of Amino Acid Transporter EAAT3 and Protects Neurons from Oxidative Stress and Epilepsy-Induced Pathology Cell Rep, 2019-03-05;26(10):2792-2804.e6. 2019-03-05 [PMID: 30840898] (ICC, IHC, Immunoprecipitation, Western Blot, Mouse)
• Joanna I. Giza, Jihye Kim, Heidi C. Meyer, Agustin Anastasia, Iva Dincheva, Crystal I. Zheng et al. The BDNF Val66Met Prodomain Disassembles Dendritic Spines Altering Fear Extinction Circuitry and Behavior Neuron 7/1/2018 [PMID: 29909994]
• K Uchiyama, M Tomita, M Yano, J Chida, H Hara, NR Das, A Nykjaer, S Sakaguchi Prions amplify through degradation of the VPS10P sorting receptor sortilin PLoS Pathog., 2017-06-30;13(6):e1006470. 2017-06-30 [PMID: 28665987] (Western Blot, Mouse)
• Q Ma, J Yang, TA Milner, JG Vonsattel, ME Palko, L Tessarollo, BL Hempstead SorCS2-mediated NR2A trafficking regulates motor deficits in Huntington's disease JCI Insight, 2017-05-04;2(9):. 2017-05-04 [PMID: 28469074] (IHC-Fr, Mouse)
• A Forge, RR Taylor, SJ Dawson, M Lovett, DJ Jagger Disruption of SorCS2 reveals differences in the regulation of stereociliary bundle formation between hair cell types in the inner ear PLoS Genet, 2017-03-27;13(3):e1006692. 2017-03-27 [PMID: 28346477] (IHC-Fr, Mouse)
• Simon Boggild, Simon Molgaard, Simon Glerup, Jens Randel Nyengaard Spatiotemporal patterns of sortilin and SorCS2 localization during organ development BMC Cell Biology 12/1/2016 [PMID: 26964886]
• Simon Boggild, Simon Molgaard, Simon Glerup, Jens Randel Nyengaard Spatiotemporal patterns of sortilin and SorCS2 localization during organ development BMC Cell Biology 2016-12-01 [PMID: 26964886] (Immunohistochemistry, Mouse)

Reviews for SorCS2 Antibody (AF4237) (5)

Average Rating: 4.4

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Mouse, Rat, Other.

reviewed by: Verified Customer WB Mouse 12/17/2014

Summary

• Application: Western Blot
• Sample Tested: Hippocampal lysates from p20 mice
• Species: Mouse

reviewed by: Verified Customer WB Rat 12/17/2014

Summary

• Application: Western Blot
• Sample Tested: Primary rat Schwann cells
• Species: Rat

reviewed by: Verified Customer IP Mouse 12/17/2014

Summary

• Application: Immunoprecipitation
• Sample Tested: IP of endogenous SorCS2 in mouse tissues as well as SorCS2 knockout mouse
• Species: Mouse

reviewed by: Verified Customer ICC Mouse 12/16/2014

Summary

• Application: Immunocytochemistry
• Sample Tested: Mouse Tissues
• Species: Mouse
• Lot: N/A

reviewed by: Verified Customer WB Other 12/16/2014

Summary

• Application: Western Blot
• Sample Tested: mouse tissue homogenates
• Species: Other
• Lot: N/A

Bioinformatics

• Gene Symbol: SORCS2
• Uniprot:
  • Q9EPR5()