Sorbitol Dehydrogenase Antibody (OTI9E7)

Western Blot: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Sorbitol Dehydrogenase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Sorbitol Dehydrogenase.

Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126] - Staining of HepG2 cells using anti-SORD mouse monoclonal antibody.

Flow Cytometry: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - HEK293T cells transfected with either pCMV6-ENTRY Sorbitol Dehydrogenase.(Red) or empty vector control plasmid (Blue) were immunostained with anti-Sorbitol Dehydrogenase mouse monoclonal, and then analyzed by flow cytometry.

Western Blot: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] Analysis of extracts (35ug) from 9 different cell lines by using anti-Sorbitol Dehydrogenase monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Sorbitol Dehydrogenase Antibody (OTI9E7) [NBP2-02126] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Sorbitol Dehydrogenase.

Flow Cytometry: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - Analysis of Hela cells, using anti-Sorbitol Dehydrogenase antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: Sorbitol Dehydrogenase Antibody (9E7) [NBP2-02126] - Analysis of Jurkat cells, using anti-Sorbitol Dehydrogenase antibody, (Red) compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI9E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.9 mg/ml

Sorbitol Dehydrogenase Antibody (OTI9E7) Summary

• Immunogen: Full length human recombinant protein of human SORD (NP_003095) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SORD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Theoretical MW: 38.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.9 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Sorbitol Dehydrogenase Antibody (OTI9E7)

• EC 1.1.1
• EC 1.1.1.14
• L-iditol 2-dehydrogenase
• sorbitol dehydrogenase
• SORD1

Background

Sorbitol dehydrogenase (SDH), a member of the medium-chain dehydrogenase/reductase protein family and the second enzyme of the polyol pathway of glucose metabolism, converts sorbitol to fructose strictly using NAD(+) as coenzyme. SDH is expressed almost ubiquitously in all mammalian tissues. The enzyme has attracted considerable interest due to its implication in the development of diabetic complications as the polyol pathway is particularly active in hyperglycemic states. Although SORD is closely related to the class I long-chain alcohol dehydrogenases, it differs in substrate specificity, catalyzing polyols such as sorbitol and xylitol but having no activity towards primary alcohols.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SORD
• Entrez:
  • 6652(Human)