Sonic Hedgehog/Shh Antibody - C-terminus


Western blot shows lysates of human stomach tissue and mouse stomach tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Sonic Hedgehog/Shh C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog more
Sonic Hedgehog was detected in immersion fixed frozen sections of mouse spinal cord using 15 µg/mL Mouse Sonic Hedgehog C-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF445) overnight at more

Product Details

Reactivity MuSpecies Glossary
Applications WB, IHC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Sonic Hedgehog/Shh Antibody - C-terminus Summary

E. coli-derived recombinant mouse Sonic Hedgehog
Cys25-Ser437 with a Lys122Arg substitution
Accession # Q62226
Detects mouse Shh C-terminal peptide in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse (rm) Dhh C-terminal peptide is observed and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N‑terminal peptide (amino acids 25-198) is observed. Detects human and mouse Sonic Hedgehog protein in Western blots. Shh C-terminal peptide specific IgG was purified by first passing the goat sera over a mouse Shh N-terminal peptide affinity column. The unbound fraction from the mouse Shh N-terminal peptide affinity column was subsequently purified using a mouse Shh C-terminal peptide affinity column.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Read Publications using
AF445 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sonic Hedgehog/Shh Antibody - C-terminus

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT


The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh). Hedgehog proteins are important signaling molecules during embryonic development. Shh genes are highly conserved and have been identified in a variety of species including human, mouse, frog, fish, and chicken. Mouse and human Shh are 92% identical at the amino acid sequence level. Shh is expressed in key embryonic tissues such as the Hensen’s node, the zone of polarizing activity in the posterior limb bud, the notochord, and the floor plate of the neural tube. Shh is involved in regulating the patterning of the developing central nervous system, somite, and limb. Shh plays an important role in the development of particular tissues such as whisker, hair, foregut, tooth and bone. Evidence also suggests that Shh is involved in regulating stem cell fates of neural and hematopoeitic lineages, and that aberrant Shh signaling is implicated in basal cell carcinomas and other diseases.

Mouse Shh cDNA encodes a 437 amino acid residue with a predicted 24 aa residue signal peptide that is cleaved to generate a 413 aa residue precursor protein. An autocatalytic reaction yields a 19 kDa amino-terminal domain Shh-N protein containing cholesterol and palmitate, and a 27 kDa carboxy-terminal domain Shh-C protein. The N-terminal domain retains all known signaling capabilities, while the C-terminal domain is responsible for the intramolecular processing, acting as a cholesterol transferase. Shh can act as both a short-range contact dependent factor and as a long-range, diffusible morphogen. At the cell surface, Shh activity is mediated by a multicomponent receptor complex involving the 12-pass transmembrane protein Patched (Ptc) which binds Shh with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor. In the absence of Shh, Ptc represses Smo activity. The binding of Shh to Ptc, releases the basal repression of Smo by Ptc.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, KD, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: BA, BA
Species: Hu
Applications: ELISA, AP, PA, WB
Species: Hu
Applications: ELISA, ICC/IF, WB
Species: Hu, Mu
Applications: BA
Species: Bt, Bv, Ca, Eq, Ha, Hu, Pm, Mu, Po, Rb, Rt
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: ChIP, ICC, WB
Species: Mu
Applications: IHC, WB
Species: Mu
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: BA
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
Species: Mu
Applications: IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P
Species: Hu
Applications: BA
Species: Hu
Applications: BA

Publications for Sonic Hedgehog/Shh Antibody (AF445)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.

Filter By Application
Flow Cytometry
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 8 of 8.
Publications using AF445 Applications Species
A Mafi, R Purohit, E Vielmas, AR Lauinger, B Lam, YS Cheng, T Zhang, Y Huang, SK Kim, WA Goddard, AE Ondrus Hedgehog proteins create a dynamic cholesterol interface PLoS ONE, 2021-02-25;16(2):e0246814. 2021-02-25 [PMID: 33630857] (Western Blot, Human) Western Blot Human
A Andreu-Cer, I Anselme, A Karam, C Laclef, M Catala, S Schneider- The ciliopathy gene ftm/rpgrip1l controls mouse forebrain patterning via region-specific modulation of hedgehog/gli signaling J. Neurosci., 2019-01-28;0(0):. 2019-01-28 [PMID: 30692221] (IHC, Mouse) IHC Mouse
Mills , Lisa D, Zhang , Yaqing, Marler , Ronald J, Herreros-Villanueva , Marta, Zhang , Lizhi, Almada , Luciana, Couch , Fergus, Wetmore , Cynthia, Pasca di Magliano , Marina, Fernandez-Zapico , Martin E Loss of the transcription factor GLI1 identifies a signaling network in the tumor microenvironment mediating KRAS oncogene-induced transformation. J Biol Chem, 2013-03-12;288(17):11786-94. 2013-03-12 [PMID: 23482563] (IHC-P, Mouse) IHC-P Mouse
Irvin DK, Jouanneau E, Duvall G T cells enhance stem-like properties and conditional malignancy in gliomas. PLoS ONE, 2010-06-07;5(6):e10974. 2010-06-07 [PMID: 20539758] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Nolan-Stevaux O, Lau J, Truitt ML, Chu GC, Hebrok M, Fernandez-Zapico ME, Hanahan D GLI1 is regulated through Smoothened-independent mechanisms in neoplastic pancreatic ducts and mediates PDAC cell survival and transformation. Genes Dev., 2009-01-01;23(1):24-36. 2009-01-01 [PMID: 19136624] (IHC-P, Mouse) IHC-P Mouse
Vue TY, Aaker J, Taniguchi A Characterization of progenitor domains in the developing mouse thalamus. J. Comp. Neurol., 2007-11-01;505(1):73-91. 2007-11-01 [PMID: 17729296] (IHC-Fr, Mouse) IHC-Fr Mouse
Feldmann G, Dhara S, Fendrich V, Bedja D, Beaty R, Mullendore M, Karikari C, Alvarez H, Iacobuzio-Donahue C, Jimeno A, Gabrielson KL, Matsui W, Maitra A Blockade of hedgehog signaling inhibits pancreatic cancer invasion and metastases: a new paradigm for combination therapy in solid cancers. Cancer Res., 2007-03-01;67(5):2187-96. 2007-03-01 [PMID: 17332349] (ICC, Human) ICC Human
Sacedon R, Varas A, Hernandez-Lopez C, Gutierrez-deFrias C, Crompton T, Zapata AG, Vicente A Expression of hedgehog proteins in the human thymus. J. Histochem. Cytochem., 2003-11-01;51(11):1557-66. 2003-11-01 [PMID: 14566027] (IHC-Fr, Human) IHC-Fr Human

Reviews for Sonic Hedgehog/Shh Antibody (AF445) (0)

There are no reviews for Sonic Hedgehog/Shh Antibody (AF445). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Sonic Hedgehog/Shh Antibody (AF445) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional Sonic Hedgehog/Shh Products

Bioinformatics Tool for Sonic Hedgehog/Shh Antibody (AF445)

Discover related pathways, diseases and genes to Sonic Hedgehog/Shh Antibody (AF445). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Sonic Hedgehog/Shh Antibody (AF445)

Discover more about diseases related to Sonic Hedgehog/Shh Antibody (AF445).

Pathways for Sonic Hedgehog/Shh Antibody (AF445)

View related products by pathway.

PTMs for Sonic Hedgehog/Shh Antibody (AF445)

Learn more about PTMs related to Sonic Hedgehog/Shh Antibody (AF445).

Blogs on Sonic Hedgehog/Shh

There are no specific blogs for Sonic Hedgehog/Shh, but you can read our latest blog posts.
mFlour Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Sonic Hedgehog/Shh Antibody - C-terminus and receive a gift card or discount.


Gene Symbol SHH