Genetic Strategies: Knockdown Validated: SODD/BAG4 Antibody [NB100-56696] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout (KO) HeLa cell line. ...read more
Western Blot: SODD/BAG4 Antibody [NB100-56696] - Analysis of 30 ug of total cell lysate from HeLa cells with anti-SODD at 2 ug/ml.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.2% gelatin
0.05% Sodium Azide
Protein G purified
Alternate Names for SODD/BAG4 Antibody
BAG-family molecular chaperone regulator-4
Bcl-2-associated athanogene 4
BCL2-associated athanogene 4
Silencer of death domains
SODDBAG family molecular chaperone regulator 4
Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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