SODD/BAG4 Antibody - BSA Free

Western Blot: SODD/BAG4 Antibody [NB100-56696] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human SODD/BAG4 Polyclonal Antibody (Catalog # NB100-56696) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SODD/BAG4 at approximately 75 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

Western Blot: SODD/BAG4 Antibody [NB100-56696] - Analysis of 30 ug of total cell lysate from HeLa cells with anti-SODD at 2 ug/ml.

• Reactivity: Hu
• Applications: WB, Flow, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

SODD/BAG4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SODD/BAG4 Antibody - BSA Free (NB100-56696) is a polyclonal antibody validated for use in WB and Flow. Anti-SODD/BAG4 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This polyclonal antibody was raised against a peptide corresponding to amino acids 443-457 of human SODD protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BAG4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface) reported in scientific literature (PMID 15592361)
  • Flow Cytometry 1:10-1:1000
  • Knockdown Validated
  • Western Blot 2-4 ug/ml
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.2% gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for SODD/BAG4 Antibody - BSA Free

• BAG4
• BAG-4
• BAG-family molecular chaperone regulator-4
• Bcl-2-associated athanogene 4
• BCL2-associated athanogene 4
• Silencer of death domains
• SODD
• SODDBAG family molecular chaperone regulator 4

Background

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SODD/BAG4 Antibody (NB100-56696)(4)

Publications using NB100-56696

• Multhoff G. Heat shock protein 70 (Hsp70): membrane location, export and immunological relevance. Methods. 2007-11-01 [PMID: 17920520] (WB, Human)
  
  Details:
  WB: Human colon and pancreatic cell lines.
• Gastpar R, Gehrmann M, Bausero MA et al. Heat shock protein 70 surface-positive tumor exosomes stimulate migratory and cytolytic activity of natural killer cells. Cancer Res. 2005-06-15 [PMID: 15958569]
• Gehrmann M, Marienhagen J, Eichholtz-Wirth H et al. Dual function of membrane-bound heat shock protein 70 (Hsp70), Bag-4, and Hsp40: protection against radiation-induced effects and target structure for natural killer cells. Cell Death Differ. 2005-01-01 [PMID: 15592361] (Flow-CS, WB, Human)
  
  Details:
  Flow cytometry (cell surface) & WB: Human colon carcinoma sublines CX+ and CX- generated from the parental cell line CX2, Nk cell line, and HeLa cell line, Table 1, 2, & 3.
• Endres R, Hacker G, Brosch I et al. Apparently normal tumor necrosis factor receptor 1 signaling in the absence of the silencer of death domains. Mol Cell Biol. 2003-09-01 [PMID: 12944486]

Bioinformatics

• Gene Symbol: BAG4
• Uniprot:
  • O95429()