SODD/BAG4 Antibody

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Genetic Strategies: Knockdown Validated: SODD/BAG4 Antibody [NB100-56696] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SODD/BAG4 knockout (KO) HeLa cell line. ...read more
Western Blot: SODD/BAG4 Antibody [NB100-56696] - Analysis of 30 ug of total cell lysate from HeLa cells with anti-SODD at 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Flow-CS, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

SODD/BAG4 Antibody Summary

Immunogen
This polyclonal antibody was raised against a peptide corresponding to amino acids 443-457 of human SODD protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BAG4
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Flow Cytometry 1:10-1:1000
  • Flow (Cell Surface)
  • Knockdown Validated
Application Notes
Flow Cytometry (Cell Surface): See Gehrmann (2005) et al. Use in Flow-cell surface reported in scientific literature (PMID: 15592361).
Control
HeLa Whole Cell Lysate
SODD/BAG4 Knockout HeLa Cell Lysate
Publications
Read Publications using
NB100-56696 in the following applications:

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for SODD/BAG4 Antibody

  • BAG4
  • BAG-4
  • BAG-family molecular chaperone regulator-4
  • Bcl-2-associated athanogene 4
  • BCL2-associated athanogene 4
  • Silencer of death domains
  • SODD
  • SODDBAG family molecular chaperone regulator 4

Background

Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

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Bioinformatics

Gene Symbol BAG4
Uniprot