Western blot shows lysates ofMCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse ...read more
SOD2/Mn‑SOD was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn‑SOD Monoclonal Antibody (Catalog # MAB3419) at 25 µg/mL for 3 hours at room ...read more
SOD2/Mn‑SOD was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn‑SOD Monoclonal Antibody (Catalog # MAB3419) at 3 µg/mL for 3 hours at room ...read more
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for SOD2/Mn-SOD Antibody (349810) [Unconjugated]
manganese-containing superoxide dismutase
Mn superoxide dismutase
superoxide dismutase [Mn], mitochondrial
superoxide dismutase 2, mitochondrial
Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2), also known as Manganese (Mn) SOD, mitochondrial SOD, and IPO-B, is an intramitochondrial 22 kDa homotetramer. Each SOD2 monomer binds one Mn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD2 shares only 23% and 17% sequence identity with SOD1 and SOD3, respectively. SOD2 is, however, well conserved from species to species and shares 90% and 87% homology with mouse and rat SOD2, respectively. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation. SOD2 is required for normal biologic function of tissues by maintaining the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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