SOD2/Mn-SOD Antibody (349810) [Unconjugated]

Images

 
Western blot shows lysates ofMCF-7 human breast cancer cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL Mouse ...read more
SOD2/Mn‑SOD was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn‑SOD Monoclonal Antibody (Catalog # MAB3419) at 25 µg/mL for 3 hours at room ...read more
SOD2/Mn‑SOD was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human/Mouse/Rat SOD2/Mn‑SOD Monoclonal Antibody (Catalog # MAB3419) at 3 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
349810
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SOD2/Mn-SOD Antibody (349810) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SOD2/Mn-SOD
Lys25-Lys222
Accession # P04179
Specificity
Detects human, mouse, and rat SOD2 in Western blots. In Western blots, no cross-reactivity with recombinant human SOD1 or SOD3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SOD2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Immunocytochemistry 1-25 ug/mL
Publications
Read Publications using
MAB3419 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD2/Mn-SOD Antibody (349810) [Unconjugated]

  • EC 1.15.1.1
  • indophenoloxidase B
  • IPOB
  • IPO-B
  • manganese-containing superoxide dismutase
  • mangano-superoxide dismutase
  • Mn SOD
  • Mn superoxide dismutase
  • MnSOD
  • MVCD6
  • SOD, Mitochodrial
  • SOD2
  • superoxide dismutase [Mn], mitochondrial
  • superoxide dismutase 2, mitochondrial

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2), also known as Manganese (Mn) SOD, mitochondrial SOD, and IPO-B, is an intramitochondrial 22 kDa homotetramer. Each SOD2 monomer binds one Mn2+ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD2 shares only 23% and 17% sequence identity with SOD1 and SOD3, respectively. SOD2 is, however, well conserved from species to species and shares 90% and 87% homology with mouse and rat SOD2, respectively. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation. SOD2 is required for normal biologic function of tissues by maintaining the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF

Publications for SOD2/Mn-SOD Antibody (MAB3419)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(6)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Porcine
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB3419 Applications Species
P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells Front Physiol, 2018;9(0):1754. 2018 [PMID: 30574096] (Western Blot, Mouse) Western Blot Mouse
JM Adrian-Seg, K Sreenivasa, P Gajawada, H Lörchner, T Braun, J Pöling The AB loop of oncostatin M (OSM) determines species-specific signaling in humans and mice J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30373773] (Western Blot, Human) Western Blot Human
S Sun, J Liu, M Zhao, Y Han, P Chen, Q Mo, B Wang, G Chen, Y Fang, Y Tian, J Zhou, D Ma, Q Gao, P Wu Loss of the novel mitochondrial protein FAM210B promotes metastasis via PDK4-dependent metabolic reprogramming Cell Death Dis, 2017;8(6):e2870. 2017 [PMID: 28594398] (Western Blot, Human) Western Blot Human
Saraf R, Huang T, Mahmood F, Owais K, Bardia A, Khabbaz K, Liu D, Senthilnathan V, Lassaletta A, Sellke F, Matyal R Early Cellular Changes in the Ascending Aorta and Myocardium in a Swine Model of Metabolic Syndrome. PLoS ONE, 2016;11(1):e0146481. 2016 [PMID: 26766185] (Western Blot, Porcine) Western Blot Porcine
Van Brussel, Ilse, Schrijvers, Dorien M, Martinet, Wim, Pintelon, Isabel, Deschacht, Maartje, Schnorbusch, Kathy, Maes, Louis, Bosmans, Johan M, Vrints, Christia, Adriaensen, Dirk, Cos, Paul, Bult, Hidde Transcript and protein analysis reveals better survival skills of monocyte-derived dendritic cells compared to monocytes during oxidative stress. PLoS ONE, 2012;7(8):e43357. 2012 [PMID: 22916248] (Western Blot, Human) Western Blot Human
Duan W, Peng Q, Masuda N, Ford E, Tryggestad E, Ladenheim B, Zhao M, Cadet JL, Wong J, Ross CA Sertraline slows disease progression and increases neurogenesis in N171-82Q mouse model of Huntington's disease. Neurobiol. Dis., 2008;30(3):312-22. 2008 [PMID: 18403212] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol SOD2
Uniprot