SOD2/Mn-SOD Antibody (349810) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: 349810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

SOD2/Mn-SOD Antibody (349810) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human SOD2/Mn-SOD
  Lys25-Lys222
  Accession # P04179
• Specificity: Detects human, mouse, and rat SOD2 in Western blots. In Western blots, no cross-reactivity with recombinant human SOD1 or SOD3 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD2/Mn-SOD Antibody (349810) [Alexa Fluor® 350]

• EC 1.15.1.1
• indophenoloxidase B
• IPOB
• IPO-B
• manganese-containing superoxide dismutase
• mangano-superoxide dismutase
• Mn SOD
• Mn superoxide dismutase
• MnSOD
• MVCD6
• SOD, Mitochodrial
• SOD2
• superoxide dismutase [Mn], mitochondrial
• superoxide dismutase 2, mitochondrial

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidases (IPOs), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 2 (SOD2), also known as Manganese (Mn) SOD, mitochondrial SOD, and IPO-B, is an intramitochondrial 22 kDa homotetramer. Each SOD2 monomer binds one Mn²⁺ ion. Three isozymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD2 shares only 23% and 17% sequence identity with SOD1 and SOD3, respectively. SOD2 is, however, well conserved from species to species and shares 90% and 87% homology with mouse and rat SOD2, respectively. Oxidative stress has been implicated in many diseases and the chief source of reactive oxygen species within the cell is the mitochondrion. SOD2 is a free radical scavenging enzyme that protects against damage from superoxide produced as a byproduct of oxidative phosphorylation. SOD2 is required for normal biologic function of tissues by maintaining the integrity of mitochondrial enzymes susceptible to inactivation by superoxide. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.