SOCS-7/Nck/NAP4 Antibody (23E4) - BSA Free

Immunohistochemistry: SOCS-7/Nck/NAP4 Antibody (23E4) [NBP3-26310] - Image of SOCS-7/Nck/NAP4 Antibody (23E4) diluted at 1:50 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.43% DAB.

Flow Cytometry: SOCS-7/Nck/NAP4 Antibody (23E4) [NBP3-26310] - Overlay Peak curve showing HepG2 cells stained with SOCS-7/Nck/NAP4 Antibody (23E4) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC
• Clone: 23E4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SOCS-7/Nck/NAP4 Antibody (23E4) - BSA Free Summary

• Description: Novus Biologicals Rabbit SOCS-7/Nck/NAP4 Antibody (23E4) - BSA Free (NBP3-26310) is a recombinant monoclonal antibody validated for use in IHC, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from Human SOCS-7/Nck/NAP4 [UniProt O14512]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SOCS7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SOCS-7/Nck/NAP4 Antibody (23E4) - BSA Free

• NAP4
• NAP-4
• NAP4SOCS-7
• Nck
• Nck, Ash and phospholipase C binding protein
• Nck, Ash and phospholipase C gamma-binding protein
• NCKAP4
• Nck-associated protein 4
• SOCS6
• SOCS7
• SOCS-7
• suppressor of cytokine signaling 7

Background

Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SOCS7