SOCS-5 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SOCS-5 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

SOCS-5 was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Goat Anti-Human SOCS-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4796) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SOCS-5 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SOCS-5
  Met1-Lys536
  Accession # O75159
• Specificity: Detects human SOCS-5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SOCS‑1, -2, -3, -6, and -7 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SOCS5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOCS-5 Antibody [Unconjugated]

• CIS6
• CIS6Cytokine-inducible SH2 protein 6
• CISH5
• CISH6
• CISH6CIS-6
• KIAA0671Cytokine-inducible SH2-containing protein 5
• SOCS5
• SOCS-5
• SOCS-5CISH5
• suppressor of cytokine signaling 5

Background

SOCS-5 (Suppressor of cytokine signaling 5; also CIS-6) is a 55‑60 kDa member of the SOCS family of proteins. It is an SH2-domain containing cytoplasmic adaptor protein expressed by Th1 cells that is known to reduce the expression of the EGF receptor and block signaling by the IL-4 R alpha chain. Human SOCS-5 is 536 amino acids (aa) in length. It contains one SH2 domain (aa 381‑476) and a SOCS box (aa 471‑520) that mediates interaction with E3 ubiquitin ligase complexes. Full-length SOCS-5 is 94% aa identical to mouse SOCS-5.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOCS-5 Antibody (AF4796)(1)

Publication using AF4796

• D.G. Walker, A.M. Whetzel, L.-F. Lue Expression of suppressor of cytokine signaling genes in human elderly and Alzheimer’s disease brains and human microglia Neuroscience 2015-08-27 [PMID: 25286386]

Bioinformatics

• Gene Symbol: SOCS5
• Uniprot:
  • O75159()