SNF8 Antibody

Images

 
Western Blot: SNF8 Antibody [NBP2-15125] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: SNF8 Antibody [NBP2-15125] - Human colon carcinoma, using SNF8 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, IHC, WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SNF8 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SNF8. The exact sequence is proprietary.
Predicted Species
Mouse (98%), Rat (99%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNF8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Application Notes
Use in immunoblotting reported in scientific literature (PMID: 31519728).
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-15125.

Reactivity Notes

Zebrafish (82%), Xenopus laevis (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for SNF8 Antibody

  • EAP30 subunit of ELL complex
  • EAP30hVps22
  • ELL-associated protein of 30 kDa
  • ESCRT-II complex subunit VPS22
  • SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae)
  • vacuolar-sorting protein SNF8
  • VPS22Dot3

Background

SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SNF8 Antibody (NBP2-15125)(1)

We have publications tested in 1 application: IB.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SNF8
Uniprot