VPS25 Antibody

Images

 
Western Blot: VPS25 Antibody [NB100-2468] - (0.5ug/ml) staining of Rat Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: VPS25 Antibody [NB100-2468] - (10ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS25 Antibody Summary

Immunogen
Peptide with sequence C-QPNVDTRQKQ corresponding to internal region according to NP_115729.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3-1ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
Western blot: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1).
IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Data obtained from the previous batch (different goat).
Positive Control
Liver Lysate (NB820-59232)
VPS25 Lysate (NBL1-17739)
Publications
Read Publication using NB100-2468.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: HUman,Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS25 Antibody

  • Dermal papilla-derived protein 9
  • DERP9
  • DERP9hVps25
  • EAP20
  • EAP20FAP20
  • ELL-associated protein of 20 kDa
  • ESCRT-II complex subunit VPS25
  • FAP20
  • MGC10540
  • vacuolar protein sorting 25 (yeast)
  • vacuolar protein sorting 25 homolog (S. cerevisiae)
  • vacuolar protein-sorting-associated protein 25

Background

Gene ID(s): 84313 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VPS25 Antibody (NB100-2468)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS25
Entrez
Uniprot