VPS25 Antibody

Images

 
Western Blot: VPS25 Antibody [NB100-2468] - Staining of Rat Kidney lysate (35 ug protein in RIPA buffer) with antibody at 0.5 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Rt, Hu, Mu, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

VPS25 Antibody Summary

Immunogen
Peptide with sequence C-QPNVDTRQKQ corresponding to internal region according to NP_115729.1.
Predicted Species
Human (100%), Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
VPS25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.3 - 1ug/mL
Application Notes
Western blot: Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1).
IHC: In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Data obtained from the previous batch (different goat).
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
VPS25 Overexpression Lysate
Publications
Read Publication using NB100-2468.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VPS25 Antibody

  • Dermal papilla-derived protein 9
  • DERP9
  • DERP9hVps25
  • EAP20
  • EAP20FAP20
  • ELL-associated protein of 20 kDa
  • ESCRT-II complex subunit VPS25
  • FAP20
  • MGC10540
  • vacuolar protein sorting 25 (yeast)
  • vacuolar protein sorting 25 homolog (S. cerevisiae)
  • vacuolar protein-sorting-associated protein 25

Background

Gene ID(s): 84313 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, PEP-ELISA

Publications for VPS25 Antibody (NB100-2468)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol VPS25
Entrez
Uniprot