SNAP23 Antibody

Images

 
Western Blot: SNAP23 Antibody [NBP1-32694] - (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000
Immunocytochemistry/ Immunofluorescence: SNAP23 Antibody [NBP1-32694] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SNAP23 Antibody Summary

Immunogen
Full length human SNAP23 Recombinant protein.
Localization
Cell membrane, Peripheral membrane protein, Cell junction, synapse, synaptosome
Predicted Species
Rhesus Macaque (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SNAP23
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (87%), Rat (88%), Pig (87%), Rabbit (88%), Chicken (82%), Bovine (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNAP23 Antibody

  • HsT17016
  • SNAP23
  • SNAP-23
  • SNAP23A
  • SNAP23B
  • synaptosomal-associated protein 23
  • synaptosomal-associated protein, 23kD
  • synaptosomal-associated protein, 23kDa
  • Vesicle-membrane fusion protein SNAP-23

Background

Synaptosomal-associated proteins (SNAPs) are cytosolic proteins that play a key role in in the process of membrane fusion in intracellular vesicle trafficking. In eukaryotic cells, the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex is critical to membrane docking and fusion and is believed to impart some degree of specificity between vesicle SNARE (v-SNARE) and target organelle SNARE (t-SNARE). In neurons and neuroendocrine cells, the SNARE complex consists of the integral membrane proteins VAMP (vesicle-associated membrane protein), syntaxin and SNAP-25. In non-neuronal tissue, a SNAP-25 homolog, SNAP-23, functionally replaces SNAP-25 in the SNARE complex. Studies show that VAMP, syntaxin and SNAP-23 are required for SNARE function and that this complex exists as a heterotrimer of the three proteins. In insulin signalling pathways, studies suggest that the translocation of GLUT4 in adipocytes requires that functional SNAP-23 be present in SNARE complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNAP23
Entrez