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SMYD2 Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-99700AF350
Alexa Fluor 405
NBP2-99700AF405
Alexa Fluor 488
NBP2-99700AF488
Alexa Fluor 532
NBP2-99700AF532
Alexa Fluor 594
NBP2-99700AF594
Alexa Fluor 647
NBP2-99700AF647
Alexa Fluor 700
NBP2-99700AF700
Alexa Fluor 750
NBP2-99700AF750
Biotin
NBP2-99700B
DyLight 350
NBP2-99700UV
DyLight 405
NBP2-99700V
DyLight 488
NBP2-99700G
DyLight 550
NBP2-99700R
DyLight 594
NBP2-99700DL594
DyLight 650
NBP2-99700C
DyLight 680
NBP2-99700FR
DyLight 755
NBP2-99700IR
FITC
NBP2-99700F
HRP
NBP2-99700H
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SMYD2 Antibody Summary

Immunogen
Produced in rabbits immunized with purified, recombinant Human SMYD2 (NP_064582.2; Met 1-His 433).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMYD2
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Purity
Affinity purified

Notes

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Alternate Names for SMYD2 Antibody

  • EC 2.1.1.43
  • Histone methyltransferase SMYD2
  • HSKM-Bzinc finger, MYND domain containing 14
  • KMT3CEC 2.1.1.-
  • Lysine N-methyltransferase 3C
  • MGC119305
  • SET and MYND domain containing 2
  • SET and MYND domain-containing protein 2
  • ZMYND14

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.
Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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(30 Publications)
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
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Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. Strong KDM1A protein expression was confirmed in the medulloblastic tumors from SmoA1-and Ptch+/A-mice relative to KDM1A expression in cerebellar tissue (CB) using western blotting of tissue lysates. I2-actin expression was used as a loading control. Image collected and cropped by Cite Ab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: LSD1 Antibody (1B2E5) [NB100-1762] - KDM1A is strongly overexpressed in human medulloblastomas, cell lines derived from them and murine medulloblastic tumors. KDM1A protein expresion was evaluated immunohistochemically in a tissue microarray of 70 medulloblastomas (MB) and 9 tissue samples of normal cerebellum (CB). Micrograph showing KDM1A-positive staining in a representative MB sample, and KDM1A-negative staining in CB, scale bar=100AI1/4m. Image collected and cropped by Cite Ab from the following publication (The KDM1A histone demethylase is a promising new target for the epigenetic therapy of medulloblastoma. Acta Neuropathol Commun (2013)) licensed under a CC-BY licence.
Western Blot: LSD1 Antibody (1B2E5) [NB100-1762] - Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.
(11 Publications)
Species: Hu, Mu, Pm
Applications: WB, Simple Western, ChIP, ELISA, IHC, IHC-P
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Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.
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Species: Hu, Mu, Rt, Ca, Eq
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SETD7/9 Antibody
NB100-56664
Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664] - Analysis of human small instestine using SETD7 antibody at 1:100.
Western Blot: SETD7/9 Antibody [NB100-56664] - Analysis of SETD7 in A) MCF7 and B) HeLa lysate using SETD7 antibody at 1:500.
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(1 Publication)
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NBP2-20428
Western Blot: SmyD1 Antibody [NBP2-20428] - Sample (50 ug of whole cell lysate) A: Mouse Heart, 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/Immunofluorescence: SmyD1 Antibody [NBP2-20428] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMYD1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: SmyD1 Antibody [NBP2-20428] - Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Species: Hu, Mu, Rt
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(JM73-63)
NBP2-75668
Western Blot: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of SMYD3 on different cell/tissue lysate using anti-Profilin 1 antibody at 1/1,000 dilution.Positive control:Lane1: Rat kidney Lane2: PC-12Lane3: Hela Lane4: Mouse spleenLane5: NIH-3T3 Lane6: Rat spleen
Immunocytochemistry/Immunofluorescence: SMYD3 Antibody (JM73-63) [NBP2-75668] - Staining SMYD3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SMYD3 Antibody (JM73-63) [NBP2-75668] - Analysis of paraffin-embedded mouse stomach tissue using anti-SMYD3 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
SETD8 Antibody
NBP1-91269
Western Blot: SETD8 Antibody [NBP1-91269] - Analysis of SETD8 in whole cell lysates of mouse proliferating neural progenitors (P) and differentiating neural stem cell (D) using anti-SETD8 antibody. Image from verified customer review.
Western Blot: SETD8 Antibody [NBP1-91269] - Western blot analysis of SETD8 in Lane 1. SETD8 overexpression vector containing C-terminal DDK tag. Lane 2. Empty vector.
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NB100-565
Knockout Validated: FUS Antibody [NB100-565] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-565) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.
Western Blot: FUS Antibody [NB100-565] - FUS activates GSK-3beta in transfected cells and transgenic mice A. Cells were transfected with either control vector (CTRL), HA-FUS, HA-FUSR521C or HA FUSR518K and the samples probed on immunoblots for GSK-3beta phosphorylated on serine 9 (GSK-3beta-S9), total GSK-3beta, FUS (using FUS antibody) and tubulin as a loading control. Phosphorylation of GSK-3beta serine 9 is the principal mechanism for regulating its activity; serine 9 phosphorylation inhibits GSK-3beta activity. Bar chart shows relative levels of GSK-3beta serine 9 phosphorylation following quantification of signals from immunoblots and normalization to total GSK-3beta signals. Data were analysed by one-way ANOVA and Tukey's post hoc test. N = 4, error bars are s.e.m.; *P < 0.05. Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/10.15252/embr.201541726), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: FUS Antibody [NB100-565] - FUS (NB100-565) (green), b-III Tubulin (white), DAPI (blue). FUS antibody dilution: 1:300 in PBST (0.1% Triton X-100) + 10% GS O/N 4C. ICC/IF image submitted by a verified customer review.
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Antibody
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Western Blot: HSP90 alpha Antibody [NBP1-77685] - Analysis of HSP901a in 1: HeLa. 2: Ntera2. 3: A431. 4: HepG2. 5: MCF7. 6: NIH/3T3. 7: PC12. 8: COS7.
Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77685] - HSP901a antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
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Histone H3
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Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.
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Carm1 Antibody
NB200-342
Knockdown Validated: Carm1 Antibody [NB200-342] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Carm1 knockout (KO) HEK293T cell line. PVDF membrane was probed with 1:5000 ug/ml of Rabbit Anti-Human Carm1 Polyclonal Antibody (Catalog # NB200-342) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Carm1 at approximately 65 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.
Western Blot: Carm1 Antibody [NB200-342] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CARM1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-342] - Detection of human CARM1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CARM1 (Cat. No. A300-421A lot 4) used at 1:1,000 (1ug/ml). Detection: DAB
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MTSS1 Antibody
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Western Blot: MTSS1 Antibody [NBP2-24716] - Analysis of MTSS1 in human testis lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse and 4) rat testis lysate using NBP2-24716 at 2 ug/ml, 4 ug/ml and 2 ug/ml, respectively.
Immunohistochemistry-Paraffin: MTSS1 Antibody [NBP2-24716] - Analysis of human colon using this antibody at 10 ug/ml.
(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P
PRMT1 Antibody
(JA11-17)
NBP2-67074
Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in NIH-3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PRMT1 Antibody (JA11-17) [NBP2-67074] - Analysis of paraffin-embedded mouse liver tissue using anti-PRMT1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/Immunofluorescence: PRMT1 Antibody (JA11-17) [NBP2-67074] - Staining PRMT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
HTRA1/PRSS11
Antibody
NBP2-23869
Western Blot: HTRA1/PRSS11 Antibody [NBP2-23869] - Analysis of HtrA1 in human A) HeLa cell lysate (1 minute exposure) and B) human 293 cell lysate (15 minute exposure) using NBP2-23869 at 1:500.
Simple Western: HTRA1/PRSS11 Antibody [NBP2-23869] - Simple Western lane view shows a specific band for htrA1 in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
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NB500-101
Knockdown Validated: CENPF Antibody [NB500-101] - Kinetochore localization of RZZ and MAD1 are independent of CENP-E and CENP-F. CENP-F depletion does not interfere with the recruitment of Zwilch. The graphs show mean intensity of one (B, C, and E), two (D and F), or three (A, G, and H) experiments; the error bars indicate S.E., and the mean values for nondepleted cells are set to 1. The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases. J Biol Chem (2018)
Western Blot: CENPF Antibody [NB500-101] - 25ug of asynchronous and mitotically blocked HeLa lysates were loaded in each respective lane.
Immunocytochemistry/Immunofluorescence: CENPF Antibody [NB500-101] - Distribution of CENP-F (red), largely associated with the mitotic spindle and a clearly distinguishable frac.
(2 Reviews)
(51 Publications)
Species: Hu, Mu, Bv, Eq
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, IHC-WhMt, KD
Histamine H4R
Antibody
NLS3775
Immunohistochemistry-Paraffin: Histamine H4R Antibody [NLS3775] - Lung, Non Small-Cell Carcinoma
Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - IHC analysis of Lymph node using this antibody at 12 ug/ ml.
Immunohistochemistry-Paraffin: Histamine H4 R Antibody [NLS3775] - Anti-HRH4 / Histamine H4 Receptor antibody IHC of human Ovary, Carcinoma and Inflammatory Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
(1 Publication)
Species: Hu, Pm
Applications: ICC/IF, IHC, IHC-P
53BP1 Antibody
NB100-304
Immunohistochemistry-Frozen: 53BP1 Antibody [NB100-304] - Irradiated cochlear spiral ganglion cells, mouse, frozen section of fixed material. Image from verified customer review.
Knockout Validated: 53BP1 Antibody [NB100-304] - 53BP1 was detected in immersion fixed HeLa cells (left) but was not detected in 53BP1 knockout Hela cells (right) using Rabbit Anti-human 53BP1 polyclonal antibody (Catalog #NB100-304) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei.
Western Blot: 53BP1 Antibody [NB100-304] - Total protein from HeLa, A431, Neuro2A, and PC12 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. The observed molecular weight for these samples are ~250 kDa and the theoretical molecular weight is 214 kDa.
(14 Reviews)
(488 Publications)
Species: Hu, Mu, Rt, Bt, Bv, Ca, Fi, Gt, Pm
Applications: WB, ChIP, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ISH, Flow-IC, KD, KO
MBD2 Antibody
NB100-81657
Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.
Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB
(2 Publications)
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, ChIP
SMYD2 Antibody
NBP2-99700
SMYD2 Antibody
Species: Hu
Applications: ELISA

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-99700AF350
Alexa Fluor 405 NBP2-99700AF405
Alexa Fluor 488 NBP2-99700AF488
Alexa Fluor 532 NBP2-99700AF532
Alexa Fluor 594 NBP2-99700AF594
Alexa Fluor 647 NBP2-99700AF647
Alexa Fluor 700 NBP2-99700AF700
Alexa Fluor 750 NBP2-99700AF750
Biotin NBP2-99700B
DyLight 350 NBP2-99700UV
DyLight 405 NBP2-99700V
DyLight 488 NBP2-99700G
DyLight 550 NBP2-99700R
DyLight 594 NBP2-99700DL594
DyLight 650 NBP2-99700C
DyLight 680 NBP2-99700FR
DyLight 755 NBP2-99700IR
FITC NBP2-99700F
HRP NBP2-99700H
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol SMYD2