SMYD2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: SMYD2 Antibody [NBP1-83186] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SMYD2 Antibody [NBP1-83186] - Staining of human uterine cervix shows moderate to strong nuclear positivity in squamous epithelial cells.

Immunohistochemistry-Paraffin: SMYD2 Antibody [NBP1-83186] - Staining of human pancreas shows weak nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: SMYD2 Antibody [NBP1-83186] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMYD2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMYD2 Antibody - BSA Free (NBP1-83186) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KLLAVKEFESHLDKLDNEKKDLIQSDIAALHHFYSKHLEFPDNDSLVVLFAQVNCNGFTIEDEELSHLGSAIFPDVALMNHSCCPNVIVTYKGTLAEVRAVQEIKP
• Predicted Species: Mouse (94%), Rat (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMYD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SMYD2 Protein (NBP1-83186PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SMYD2 Antibody - BSA Free

• EC 2.1.1.43
• Histone methyltransferase SMYD2
• HSKM-B
• HSKM-Bzinc finger, MYND domain containing 14
• KMT3C
• KMT3CEC 2.1.1.-
• Lysine N-methyltransferase 3C
• MGC119305
• SET and MYND domain containing 2
• SET and MYND domain-containing protein 2
• SMYD2
• ZMYND14

Background

SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMYD2